Anticorps Recombinant de lapin anti-IL-7Ra/CD127

IL-7Ra/CD127 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

240749H2

N° de cat : 98074-1-RR

Synonymes

CD127, 240749H2, IL 7RA, IL 7R subunit alpha, IL 7R alpha

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Galerie de données de validation

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  FC experiment of human PBMCs using 98074-1-RR

  1x10^6 human PBMCs were stained with CoraLite® Plus 647 Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (CL647-65569, Clone: OKT3) and 0.25 ug Anti-Human IL-7Ra/CD127 Rabbit Recombinant Antibody (98074-1-RR, Clone:240749H2), or Isotype Control. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98074-1-RR

  1x10^6 human PBMCs were stained with 0.25 ug Anti-Human IL-7Ra/CD127 Rabbit Recombinant Antibody (98074-1-RR, Clone:240749H2) (red), or Isotype Control(blue). Cells were not fixed.

• 

  FC experiment of human PBMCs using 98074-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human IL-7Ra/CD127 Rabbit Recombinant Antibody (98074-1-RR, Clone:240749H2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), and 0.25 ug CoraLite® Plus 647 Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (CL647-65569, Clone: OKT3), and 0.25 ug Isotype Control. Cells were not fixed.

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  Affinity and Kinetic Characterization of 98074-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98074-1-RR against Human IL-7Ra/CD127 were performed. The affinity constant is 4.57 nM.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98074-1-RR cible IL-7Ra/CD127 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 7 receptor
• Masse moléculaire calculée: 52 kDa
• Numéro d’acquisition GenBank: AAC83204.1
• Symbole du gène: CD127
• Identification du gène (NCBI): 3575
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

CD127, also known as IL-7R subunit alpha (IL-7RA), is a type I membrane glycoprotein expressed on thymocytes, B cell precursors, most T cells, and some lymphoid and myeloid cells. IL-7R is a heterodimer composed of CD127 and the common-γ chain receptor (CD132). IL-7R plays critical roles in lymphocyte development and homeostasis. CD127 can also act as a receptor for thymic stromal lymphopoietin (TSLP).

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