Anticorps Recombinant de lapin anti-IL-7Ra/CD127

IL-7Ra/CD127 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

242852B4

N° de cat : 98385-1-RR

Synonymes

CD127, Il7r, IL 7Ralpha, IL- 7Ralpha, IL-7 receptor subunit alpha

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Galerie de données de validation

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  FC experiment of mouse splenocytes using 98385-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse IL-7Ra/CD127 Rabbit RecAb (98385-1-RR, Clone:242852B4) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9). Cells were then stained with APC Anti-Mouse CD3 (17A2) (APC-65077, Clone: 17A2). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98385-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse IL-7Ra/CD127 Rabbit RecAb (98385-1-RR, Clone:242852B4) (red) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98385-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse IL-7Ra/CD127 Rabbit RecAb (98385-1-RR, Clone:242852B4) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug PE Anti-Mouse IL-7Ra/CD127 (A7R34) (PE-65093, Clone: A7R34). Cells were then stained with APC Anti-Mouse CD3 (17A2) (APC-65077, Clone: 17A2). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98385-1-RR cible IL-7Ra/CD127 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IL-7Ra/CD127 Protéine recombinante Eg1826
• Nom complet: interleukin 7 receptor
• Masse moléculaire calculée: 52 kDa
• Numéro d’acquisition GenBank: NM_008372.4
• Symbole du gène: CD127
• Identification du gène (NCBI): 16197
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

CD127, also known as IL-7R subunit alpha (IL-7Rα), is a type I membrane glycoprotein expressed on thymocytes, B cell precursors, most T cells, and some lymphoid and myeloid cells (PMID: 8415665). IL-7R is a heterodimer composed of CD127 and IL-2Rγ (CD132), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15 (PMID: 8266077; 9862091). IL-7R plays critical roles in lymphocyte development and homeostasis (PMID: 26336149). CD127 can also act as a receptor for thymic stromal lymphopoietin (TSLP) (PMID: 10974032).

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