Anticorps Monoclonal anti-IL-9

IL-9 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F3A11

N° de cat : 66144-1-PBS

Synonymes

IL9, 1F3A11, IL 9, interleukin 9, interleukin9

---

Galerie de données de validation

• 

  WB analysis of Recombinant protein using 66144-1-Ig (same clone as 66144-1-PBS)

  Recombinant protein were subjected to SDS PAGE followed by western blot with 66144-1-Ig (IL9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66144-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66144-1-Ig (same clone as 66144-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66144-1-Ig (IL-9 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66144-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66144-1-Ig (same clone as 66144-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66144-1-Ig (IL-9 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66144-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 66144-1-Ig (same clone as 66144-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-9 antibody (66144-1-Ig, Clone: 1F3A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66144-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66144-1-PBS cible IL-9 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IL-9 Protéine recombinante Ag21435
• Nom complet: interleukin 9
• Masse moléculaire calculée: 144 aa, 16 kDa
• Poids moléculaire observé: 19 kDa
• Numéro d’acquisition GenBank: BC066284
• Symbole du gène: IL9
• Identification du gène (NCBI): 3578
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin 9 (IL-9) is a γc-family cytokine that is highly produced by T-helper 9 (Th9) cells. IL-9 could promote the survival and activation of various cellular targets, including mast cells, B cells, T cells, and structural cells. Its expression is considered a hallmark of Th2-lineage cells. Primarily studied in Th2-type immunity, IL-9 was shown to be involved in asthma, allergy, and host defense against helminth infections. IL-9 also stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes.(PMID: 38864109; PMID: 29038115; PMID: 9505195; PMID: 29703631)

{{ptg:RelatedPrimaryAntibodies}}