Anticorps Recombinant de lapin anti-IL-1 alpha

IL-1 alpha Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

240431G10

N° de cat : 98052-1-PBS

Synonymes

IL1-alpha, Il1a, IL-1,IL1, IL1 alpha, Il 1a

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Galerie de données de validation

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  FC experiment of RAW 264.7 using 98052-1-RR (same clone as 98052-1-PBS)

  1x10^6 LPS treated RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse IL1-alpha Rabbit Recombinant Antibody (98052-1-RR, Clone:240431G10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug Isotype Control and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 98052-1-PBS in a different storage buffer formulation.

• 

  FC experiment of RAW 264.7 using 98052-1-RR (same clone as 98052-1-PBS)

  1x10^6 LPS treated (red) or untreated (black) RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse IL1-alpha Rabbit Recombinant Antibody (98052-1-RR, Clone: 240431G10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 98052-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98052-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98052-1-RR against Mouse IL-1 alpha were performed. The affinity constant is 0.495 nM.

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Informations sur le produit

98052-1-PBS cible IL-1 alpha dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 1 alpha
• Masse moléculaire calculée: 31kd
• Numéro d’acquisition GenBank: NM_010554
• Symbole du gène: Il1a
• Identification du gène (NCBI): 16175
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

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