Anticorps Recombinant de lapin anti-IL-12RB1/CD212

IL-12RB1/CD212 Recombinant Antibody for IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241661D7

N° de cat : 84391-4-PBS

Synonymes

IL12RB1, 241661D7, CD212, IL 12R beta 1, IL 12R subunit beta 1

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Galerie de données de validation

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  Sandwich ELISA sample test of MP01262-2

  The mean IL12RB1 concentration was determined to be 27.14 ng/mL in Jurkat cell extract based on a 1.9 mg/mL extract load and 48.02 ng/mL in THP-1 cell extract based on a 1.5 mg/mL extract load.

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  Sandwich ELISA standard curve of MP01262-2

  Sandwich ELISA standard curve of MP01262-2, Human IL12RB1 Recombinant Matched Antibody Pair - PBS only. 84391-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP00005. 84391-4-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

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  IF Staining of K-562 using 84391-4-RR (same clone as 84391-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using IL12RB1 antibody (84391-4-RR, Clone: 241661D7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84391-4-PBS in a different storage buffer formulation.

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  IF Staining of THP-1 using 84391-4-RR (same clone as 84391-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using IL12RB1 antibody (84391-4-RR, Clone: 241661D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84391-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01262-2

  Cytometric bead array standard curve of MP01262-2, IL12RB1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84391-3-PBS. Detection antibody: 84391-4-PBS. Standard: RP00005. Range: 0.781-50 ng/mL.

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  Affinity and Kinetic Characterization of 84391-4-PBS

  Biolayer interferometry (BLl) kinetic assay of 84391-4-PBS against Human IL-12RB1/CD212 was performed. The affinity constant is 51.5 nM.

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Informations sur le produit

84391-4-PBS cible IL-12RB1/CD212 dans les applications de IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 12 receptor, beta 1
• Masse moléculaire calculée: 662 aa, 73 kDa
• Numéro d’acquisition GenBank: BC029121
• Symbole du gène: IL12RB1
• Identification du gène (NCBI): 3594
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IL12RB1, also named as Interleukin-12 receptor subunit beta-1 or CD212, is a 662 amino acid protein, which contains 5 fibronectin type-III domains and belongs to the type I cytokine receptor family. Type 2 subfamily. IL12RB1 localizes in the membrane and functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.

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