Anticorps Monoclonal anti-IL-17A

• Phare
• Validé par KD/KO

IL-17A Monoclonal Antibody for WB, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(34)

CloneNo.

1B3D5

Avis (1)

N° de cat : 66148-1-Ig

Synonymes

IL 17, IL17, IL-17, IL17A, 1B3D5

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Galerie de données de validation

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  WB analysis using 66148-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66148-1-Ig (IL-17A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 66148-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66148-1-Ig (IL-17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66148-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66148-1-Ig (IL-17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 66148-1-Ig

  U-937 cells were subjected to SDS PAGE followed by western blot with 66148-1-Ig (IL-17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of human tonsillitis using 66148-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-17A antibody (66148-1-Ig, Clone: 1B3D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human tonsillitis using 66148-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-17A antibody (66148-1-Ig, Clone: 1B3D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules Daudi, cellules HL-60, cellules Jurkat, cellules MOLT-4, cellules Raji, cellules Ramos, cellules RAW 264.7, cellules U-937
• Résultats positifs en IF-P: tissu d'amygdalite humain,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 25 publications below
• IHC: See 5 publications below
• IF: See 9 publications below

Informations sur le produit

66148-1-Ig cible IL-17A dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IL-17A Protéine recombinante Ag3733
• Nom complet: interleukin 17A
• Masse moléculaire calculée: 155 aa, 18 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC067505
• Symbole du gène: IL-17A
• Identification du gène (NCBI): 3605
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IL17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased.

Publications

Species	Application	Title
mouse	IF	Clin Immunol; Potential therapeutic effect of dimethyl fumarate on Treg/Th17 cell imbalance in biliary atresia; Authors - Mengting Liu
mouse	WB,IHC	Int J Pharm; Microneedle system carrying Momordin Ic-loaded ROS-responsive hydrogel ameliorates psoriasis via targeted anti-inflammatory and reactive oxygen species (ROS)-scavenging mechanisms; Authors - Chang Wang
mouse	WB	Mol Nutr Food Res; A High MCT-Based Ketogenic Diet Suppresses Th1 and Th17 Responses to Ameliorate Experimental Autoimmune Encephalomyelitis in Mice by Inhibiting GSDMD and JAK2-STAT3/4 Pathways; Authors - Qianye Zhang
mouse	IHC	J Integr Med; Ethanol extract of Herpetospermum caudigerum Wall ameliorates psoriasis-like skin inflammation and promotes degradation of keratinocyte-derived ICAM-1 and CXCL9; Authors - Ya Zhong
mouse	WB	Int Immunopharmacol; (RS)-bambuterol and its enantiomers: Potential improvement of (R)-bambuterol in mice with colitis.; Authors - Liangjun Deng
human	WB	Int Immunopharmacol; IL-17A drives cognitive aging probably via inducing neuroinflammation and theta oscillation disruption in the hippocampus.; Authors - Yachun Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Rosa (Verified Customer) (07-08-2022)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:150
• Cell Tissue Type: adipose tissue

IL-17A Antibody Immunofluorescence validation (1:150 dilution) in adipose tissue (Cat no:66148-1-Ig)

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