Anticorps Recombinant de lapin anti-IL-1R1

IL-1R1 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240130A3

N° de cat : 83420-1-PBS

Synonymes

IL1R1, IL 1R 1, EC:3.2.2.6, D2S1473, CD121A

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Galerie de données de validation

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  WB analysis using 83420-1-RR (same clone as 83420-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83420-1-RR (IL1R1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83420-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83420-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83420-1-RR against Human IL1R1 were performed. The affinity constant is 8.43 nM.

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Informations sur le produit

83420-1-PBS cible IL-1R1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IL-1R1 Protéine recombinante Ag12602
• Nom complet: interleukin 1 receptor, type I
• Masse moléculaire calculée: 569 aa, 65 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC075063
• Symbole du gène: IL1R1
• Identification du gène (NCBI): 3554
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IL-1R1 (IL-1 receptor type 1, also known as p80, IL-1R-alpha or CD121a) is a 80 kDa transmembrane glycoprotein and a member of the interleukin-1 receptor family (PMID: 35163653; 29248002). It is a receptor for IL-1α, IL-1β, and interleukin-1 receptor antagonist (IL-1Ra). IL-1α and IL-1β interact with the extracellular domain of IL-1R1, triggering the recruitment of an accessory receptor, the IL-1RAcP, resulting in a functional receptor complex that initiates IL-1R1 signaling cascades (PMID: 35163653). IL-1Ra competes with active IL-1 and blocks binding to their common activating receptor IL-1R1 and, thus, inhibits IL-1 signaling (PMID: 35332327; 11641511). It has been reported that IL-1R1 may also bind IL-38 (PMID: 29248002). IL-1R1 expression has been found in many cell types including T cells, macrophages, neutrophils, eosinophils, basophils, mast cells, fibroblasts, and endothelial cells. IL-1R1 can be released from the cell surface following proteolytic cleavage, generating a soluble form of 55-60 kDa (PMID: 17324958).

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