Anticorps Polyclonal de lapin anti-IL-1RAP

IL-1RAP Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

Avis (1)

N° de cat : 30966-1-AP

Synonymes

IL1RAP, IL-1 receptor accessory protein, IL 1RAcP, IL 1R3, IL 1R 3

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Galerie de données de validation

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  WB analysis using 30966-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30966-1-AP (IL-1RAP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 30966-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30966-1-AP (IL-1RAP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 30966-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30966-1-AP (IL-1RAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 30966-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30966-1-AP (IL-1RAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 30966-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30966-1-AP (IL-1RAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 30966-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30966-1-AP (IL-1RAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 30966-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30966-1-AP (IL-1RAP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 30966-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30966-1-AP (IL-1RAP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu hépatique de souris, tissu placentaire humain
• Résultats positifs en IHC: tissu placentaire humain, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

30966-1-AP cible IL-1RAP dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IL-1RAP Protéine recombinante Ag34121
• Nom complet: interleukin 1 receptor accessory protein
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: NM_001167928.1
• Symbole du gène: IL1RAP
• Identification du gène (NCBI): 3556
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IL-1RAP (Interleukin-1 receptor accessory protein), also known as IL-1RAcP, IL-1R-3, or IL-1R3, is a member of the interleukin-1 receptor family. IL-1RAP is composed of an extracellular region containing three Ig-like C2 type domains, a transmembrane region, and a cytoplasmic region with a TIR (Toll-IL-1-receptor) domain. IL-1RAP functions as a co-receptor for IL-1, IL-33, IL-36. It plays an essential role in the signaling of the IL-1 family cytokines such as IL-1, IL-33, and IL-36, as well as tyrosine kinases FLT3 and c-Kit. IL-1RAP can also exist in a soluble form.

Publications

Species	Application	Title
human	IHC	Front Oncol; Integrated single-cell and transcriptome sequencing data reveal the value of IL1RAP in gastric cancer microenvironment and prognosis; Authors - Weifeng Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alina (Verified Customer) (07-31-2025)

The Antibody had a strong signal, but it showed a second allegedly unspecific band. The Antibody was compared to another IL1RAP Antibody, that had a weaker signal but didn´t show an unspecific second band on the membrane. But all in all it is a good strong binding antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500

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