Anticorps Monoclonal anti-IL-2

IL-2 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain et plus (3)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(11)

CloneNo.

2F11C5

Avis (1)

N° de cat : 60306-1-Ig

Synonymes

IL2, 2F11C5, IL 2, interleukin 2, Interleukin-2

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Galerie de données de validation

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  WB analysis using 60306-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60306-1-Ig (IL-2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 60306-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60306-1-Ig (IL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 60306-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60306-1-Ig (IL2 Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

• 

  IHC staining of human tonsillitis using 60306-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60306-1-Ig (IL2 Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

• 

  IHC staining of human breast cancer using 60306-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60306-1-Ig (IL2 Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

• 

  IHC staining of human breast cancer using 60306-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60306-1-Ig (IL2 Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

• 

  IF Staining of human tonsillitis using 60306-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using IL-2 antibody (60306-1-Ig, Clone: 2F11C5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Jurkat using 60306-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using IL-2 antibody (60306-1-Ig, Clone: 2F11C5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules MOLT-4, leucocyte de sang périphérique humain
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu d'amygdalite humain,
• Résultats positifs en IF/ICC: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 4 publications below

Informations sur le produit

60306-1-Ig cible IL-2 dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, porc, souris, Hamster
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IL-2 Protéine recombinante Ag10199
• Nom complet: interleukin 2
• Masse moléculaire calculée: 153 aa, 18 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC070338
• Symbole du gène: IL2
• Identification du gène (NCBI): 3558
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IL2 is a secreted cytokine involved in the operation and regulation of immune system. IL2 plays a pivotal role in T-cell activation and effector functions, including T cell proliferation, interferon-γproduction and cytotoxicity. IL2 can stimulate the propagation of lymphocytes and induce the cytotoxic T lymphocytes and lymphokine-activated killer cells for tumor cells. IL2 also influences memory T cell homeostasis through the regulation of memory T cell numbers and enhances major histocompatibility complex II (MHC II) expression on tumor cells.

Publications

Species	Application	Title
mouse	WB	Transl Psychiatry; A diet high in sugar and fat influences neurotransmitter metabolism and then affects brain function by altering the gut microbiota.; Authors - Yinrui Guo
mouse	WB	PLoS One; N-Acetylcysteine Attenuates Ischemia-Reperfusion-Induced Apoptosis and Autophagy in Mouse Liver via Regulation of the ROS/JNK/Bcl-2 Pathway.; Authors - Chengfen Wang
mouse	WB	Inflammation; Links Between Gut Dysbiosis and Neurotransmitter Disturbance in Chronic Restraint Stress-Induced Depressive Behaviours: the Role of Inflammation; Authors - Hai‑long Yang
pig	WB,IHC	Food Addit Contam Part A Chem Anal; FumDSB can alleviate the inflammatory response induced by fumonisin B1 in growing pigs.; Authors - Quancheng Liu
human	IHC	J Clin Exp Hematop; Role of TGF-beta1 and TNF-alpha1 produced by neoplastic cells in the pathogenesis of fibrosis in patients with hematologic neoplasms; Authors - Haruko Takizawa
human	WB	Front Immunol; PTPRC promoted CD8+ T cell mediated tumor immunity and drug sensitivity in breast cancer: based on pan-cancer analysis and artificial intelligence modeling of immunogenic cell death-based drug sensitivity stratification; Authors - Pengping Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alex (Verified Customer) (10-11-2019)

IL-2 Staining is dirty in mouse colon, attempted various dilutions and retrieval methods none of which worked.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse Colon

IL-2 Antibody Immunohistochemistry, validation (1:2000 dilution) in Mouse Colon (Cat no:60306-1-Ig)

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