Anticorps Recombinant de lapin anti-IL-2

IL-2 Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

240264B2

N° de cat : 82626-2-PBS

Synonymes

Il2, interleukin 2

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Galerie de données de validation

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  Sandwich ELISA standard curve of MP00535-3

  Sandwich ELISA standard curve of MP00535-3, rat IL-2 Recombinant Matched Antibody Pair - PBS only. 82626-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0260. 82626-5-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

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  Sample test of MP00535-3

  Rat spleen, cut into 1-2 mm pieces, was cultured for 3-4 daysin 25-30 mL of RPMIsupplemented with 10% fetal bovine serum and stimulated with 10 μg/mL ConA. The cell culture supernate wasremoved, assayed for rat IL-2, and measured 45,596.05 pg/mL.

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  Sample test of MP00535-3

  Serum of seven rats was measured. The IL-2 concentration of detected samples was determined to be 95.71 pg/mL with a range of ND - 179.2 pg/mL.

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  Affinity and Kinetic Characterization of 82626-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 82626-2-PBS against Rat IL-2 was performed. The affinity constant is 1.72 nM.

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Informations sur le produit

82626-2-PBS cible IL-2 dans les applications de Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons rat

• Réactivité: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IL-2 Protéine recombinante Eg0260
• Nom complet: interleukin 2
• Masse moléculaire calculée: 18 kDa
• Numéro d’acquisition GenBank: NM_053836
• Symbole du gène: Il2
• Identification du gène (NCBI): 116562
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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