Anticorps Recombinant de lapin anti-IL-2

IL-2 Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

240264C7

N° de cat : 98046-1-PBS

Synonymes

Il2, interleukin 2, 240264C7

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Galerie de données de validation

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  FC experiment of rat splenocytes using 98046-1-RR (same clone as 98046-1-PBS)

  1x10^6 unstimulated or PMA, Ionomycin and Brefeldin A treated rat splenocytes were intracellularly stained with APC Anti-Rat CD4 (OX-35) and 0.25 ug Anti-Rat IL-2 Rabbit Recombinant Antibody (98046-1-RR, Clone: 240264C7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98046-1-PBS in a different storage buffer formulation.

• 

  FC experiment of rat splenocytes using 98046-1-RR (same clone as 98046-1-PBS)

  1x10^6 PMA, Ionomycin and Brefeldin A treated rat splenocytes were intracellularly stained with 0.25 ug Anti-Rat IL-2 Rabbit Recombinant Antibody (98046-1-RR, Clone: 240264C7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98046-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98046-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98046-1-RR against Rat IL2 were performed. The affinity constant is 0.36 nM.

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Informations sur le produit

98046-1-PBS cible IL-2 dans les applications de FC (Intra) et montre une réactivité avec des échantillons rat

• Réactivité: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 2
• Masse moléculaire calculée: 18 kDa
• Numéro d’acquisition GenBank: NM_053836
• Symbole du gène: Il2
• Identification du gène (NCBI): 116562
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

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