Anticorps Monoclonal anti-IL-23 p19

IL-23 p19 Monoclonal Antibody for IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(6)

CloneNo.

1D5D3

N° de cat : 66196-1-Ig

Synonymes

IL23A, IL-23 subunit alpha, IL 23p19, IL 23A, IL 23 subunit alpha

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Galerie de données de validation

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  IHC staining of human stomach cancer using 66196-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66196-1-Ig (IL-23A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 66196-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66196-1-Ig (IL-23A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 66196-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66196-1-Ig (IL23A Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 66196-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66196-1-Ig (IL23A Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• ELISA: See 1 publications below

Informations sur le produit

66196-1-Ig cible IL-23 p19 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IL-23 p19 Protéine recombinante Ag19406
• Nom complet: interleukin 23, alpha subunit p19
• Masse moléculaire calculée: 189 aa, 21 kDa
• Numéro d’acquisition GenBank: BC066268
• Symbole du gène: IL23A
• Identification du gène (NCBI): 51561
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin 23 (IL-23) is a member of the IL12 cytokine family and composed of two subunits, IL12p40 and IL23p19. It is produced by antigen presenting cells and has been shown to promote the production and survival of a distinct lineage of T-cells called TH17 cells. A functional receptor for IL-23 (the IL-23 receptor) has been identified and is composed of Il-12Rβ1 and IL-23R. IL-23 is expressed chiefly by the macrophages and DCs. The IL-23R is found on memory T cells, NKT cells, macrophages, DCs, and naive T cells upon activation by TGF-βand IL-6. The main biological effects of IL-23 identified initially consist of stimulation of antigen presentation by DCs, T cell differentiation to Th17 cells, and production of interferon-γ (IFN-γ). IL-23 acts also as an end-stage effector cytokine through direct action on macrophages.

Publications

Species	Application	Title
human	IHC	Thyroid; A MULTICENTER, SINGLE-BLIND, CASE-CONTROL, IMMUNOHISTOCHEMICAL STUDY OF ORBITAL TISSUE IN THYROID EYE DISEASE; Authors - YuanPing Hai
human		J Immunol; Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues; Authors - Teresa W-M Fan
human	WB,IF	Cancer Lett; PDE4D/cAMP/IL-23 axis determines the immunotherapy efficacy of lung adenocarcinoma via activating the IL-9 autocrine loop of cytotoxic T lymphocytes; Authors - Bing Feng
human	IHC	Sci Rep; Chemotherapy-induced high expression of IL23A enhances efficacy of anti-PD-1 therapy in TNBC by co-activating the PI3K-AKT signaling pathway of CTLs; Authors - Fan Pan
mouse	ELISA	Toxicol Res (Camb); Zerumbone attenuates the excessive proliferation of keratinocytes in psoriasis mice through regulating NLRP3/NF-κB pathway; Authors - Jin Lin
human	WB	Gut; IL23 induces IL23R recycling and amplifies innate receptor-induced signalling and cytokines in human macrophages, and the IBD-protective IL23R R381Q variant modulates these outcomes.; Authors - Rui Sun

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