Anticorps Recombinant de lapin anti-CD25/IL-2RA

CD25/IL-2RA Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240906A12

N° de cat : 83896-1-PBS

Synonymes

CD25, 240906A12, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha

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Galerie de données de validation

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  WB analysis of MJ using 83896-1-RR (same clone as 83896-1-PBS)

  MJ cells were subjected to SDS PAGE followed by western blot with 83896-1-RR (IL2RA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83896-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83896-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83896-1-RR against Human CD25/IL2RA were performed. The affinity constant is 31.7 nM.

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Informations sur le produit

83896-1-PBS cible CD25/IL-2RA dans les applications de WB, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD25/IL-2RA Protéine recombinante Eg0408
• Nom complet: interleukin 2 receptor, alpha
• Masse moléculaire calculée: 272 aa, 31 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC156627
• Symbole du gène: IL2RA
• Identification du gène (NCBI): 3559
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present in activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+ Foxp3+ Treg cells (PMID: 22585674).

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