Anticorps Recombinant de lapin anti-CD25/IL-2RA

CD25/IL-2RA Recombinant Antibody for Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240906D7

N° de cat : 83896-6-PBS

Synonymes

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Galerie de données de validation

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  Sandwich ELISA sample test of MP00837-3

  Serum of eight individual healthy human donors was measured. The human CD25/IL-2RA concentration of detected samples was determined to be 993.9 pg/mL with a range of 567.3 - 1,718.0 pg/mL.

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  Sandwich ELISA sample test of MP00837-3

  Human peripheral blood mononuclear cells (5 x 10^6 cells/mL) were cultured in DMEM supplemented with 5% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. The cells were cultured unstimulated or stimulated with 10 μg/mL PHA for 5 days. Aliquots of the cell culture supernates were removed and assayed for levels of human CD25/IL-2RA.

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  Sandwich ELISA standard curve of MP00837-3

  Sandwich ELISA standard curve of MP00837-3, Human CD25/IL-2RA Recombinant Matched Antibody Pair - PBS only. 83896-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0408. 83896-6-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL

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  Affinity and Kinetic Characterization of 83896-6-PBS

  Biolayer interferometry (BLl) kinetic assay of 83896-6-PBS against Human CD25/IL-2RA was performed. The affinity constant is 0.853 nM.

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Informations sur le produit

83896-6-PBS cible CD25/IL-2RA dans les applications de Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD25/IL-2RA Protéine recombinante Eg0408
• Nom complet: interleukin 2 receptor, alpha
• Masse moléculaire calculée: 272 aa, 31 kDa
• Numéro d’acquisition GenBank: BC156627
• Symbole du gène: IL2RA
• Identification du gène (NCBI): 3559
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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