Anticorps Polyclonal de lapin anti-CD122/IL-2RB

CD122/IL-2RB Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 13602-1-AP

Synonymes

CD122, CD122/IL-2RB, IL 2 receptor subunit beta, IL 2R subunit beta, IL 2RB, IL2RB, IL-2RB, interleukin 2 receptor, beta, P70 75, p75

---

Galerie de données de validation

• 

  WB analysis using 13602-1-AP

  NK-92 cells were subjected to SDS PAGE followed by western blot with 13602-1-AP (CD122/IL-2RB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse spleen using 13602-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 13602-1-AP (CD122/IL-2RB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 13602-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 13602-1-AP (CD122/IL-2RB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: NK-92 cells,
• Résultats positifs en IHC: tissu splénique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

13602-1-AP cible CD122/IL-2RB dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD122/IL-2RB Protéine recombinante Ag3929
• Nom complet: interleukin 2 receptor, beta
• Masse moléculaire calculée: 551 aa, 61 kDa
• Poids moléculaire observé: 70-85 kDa
• Numéro d’acquisition GenBank: BC025691
• Symbole du gène: IL2RB
• Identification du gène (NCBI): 3560
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The interleukin 2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. The beta subunit (IL2RB, also known as CD122 and p75) is involved in receptor mediated endocytosis and transduces the mitogenic signals of nterleukin 2.

Publications

Species	Application	Title
mouse	WB	Int J Biol Macromol; Activation of IL-2/IL-2R pathway by Hedyotis diffusa polysaccharide improves immunotherapy in colorectal cancer; Authors - Liangliang Bai
Human	WB	Allergol Immunopathol (Madr); IL2RB affects Th1/Th2 and Th17 responses of peripheral blood mononuclear cells from septic patients; Authors - Jiaqian Zhou
mouse	IF	Ocul Surf; Conditional deletion of CD25 in the corneal epithelium reveals sex differences in barrier disruption; Authors - Anmar Abu-Romman
human	WB	J Dermatol Sci; Exosomes containing miR-1469 regulate natural killer cells by targeting CD122 in non-segmental vitiligo; Authors - Yujia Wei
rat	WB	ACS Omega; Vanillic Acid Protects PC12 Cells from Corticosterone-Induced Neurotoxicity via Regulating Immune and Metabolic Dysregulation Based on Computational Metabolomics; Authors - Tianxing Lv

{{ptg:RelatedPrimaryAntibodies}}