Anticorps Polyclonal de lapin anti-IL-3

IL-3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 17835-1-AP

Synonymes

Hematopoietic growth factor, IL 3, IL3, IL-3, Interleukin 3, Mast cell growth factor, MCGF, MULTI CSF, P cell stimulating factor

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Galerie de données de validation

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  WB analysis of Recombinant protein using 17835-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 17835-1-AP (IL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Recombinant protein using 17835-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 17835-1-AP (IL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Recombinant protein using 17835-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 17835-1-AP (IL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 17835-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17835-1-AP (IL3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 17835-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17835-1-AP (IL3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: Protéine recombinante, protéine protéine recombinante
• Résultats positifs en IHC: tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

17835-1-AP cible IL-3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IL-3 Protéine recombinante Ag11839
• Nom complet: interleukin 3 (colony-stimulating factor, multiple)
• Masse moléculaire calculée: 152 aa, 17 kDa
• Poids moléculaire observé: 20 kDa
• Numéro d’acquisition GenBank: BC066273
• Symbole du gène: IL-3
• Identification du gène (NCBI): 3562
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-3 (IL-3) is a multilineage hematopoietic growth factor that promotes the proliferation, differentiation and survival of early multilineage hematopoietic progenitors. In particular, this cytokine plays a key role in stimulating the proliferation and survival of myeloid precursors. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders (PMID: 24103757;2698876;2809196)

Publications

Species	Application	Title
pig	WB	JTCVS Open; Intramyocardial injection of hypoxia-conditioned extracellular vesicles modulates apoptotic signaling in chronically ischemic myocardium; Authors - Dwight D Harris

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