Anticorps Polyclonal de lapin anti-IL-33

• Phare
• Validé par KD/KO

IL-33 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(38)

N° de cat : 12372-1-AP

Synonymes

IL33, 109-270, 95-270, 99-270, C9orf26

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Galerie de données de validation

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  WB analysis using 12372-1-AP

  PMA, LPS, and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 12372-1-AP (IL-33 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12372-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12372-1-AP (IL-33 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Recombinant protein using 12372-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 12372-1-AP (IL33 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HUVEC, cellules HeLa, cellules THP-1 traitées au PMA, LPS et à la bréfeldine A, Protéine recombinante

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 22 publications below
• IHC: See 21 publications below
• IF: See 10 publications below
• ELISA: See 2 publications below

Informations sur le produit

12372-1-AP cible IL-33 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IL-33 Protéine recombinante Ag2957
• Nom complet: interleukin 33
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC047085
• Symbole du gène: IL-33
• Identification du gène (NCBI): 90865
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IL-33 is an IL-1 family cytokine and nuclear alarmin, is constitutively expressed in epithelial barrier tissues and human blood vessels. IL-33 is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. It also acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury.

Publications

Species	Application	Title
mouse	WB	Adv Healthc Mater; Ultrasmall Nanoparticles Regulate Immune Microenvironment by Activating IL-33/ST2 to Alleviate Renal Ischemia-Reperfusion Injury; Authors - Liyao Xu
human	IHC,IF	J Invest Dermatol; Increased Type 2 Innate Lymphoid Cells in Patients with Drug Reaction with Eosinophilia and Systemic Symptoms Syndrome.; Authors - Yi-Giien Tsai
rat	WB	Front Immunol; Emodin Alleviates Sodium Taurocholate-Induced Pancreatic Acinar Cell Injury via MicroRNA-30a-5p-Mediated Inhibition of High-Temperature Requirement A/Transforming Growth Factor Beta 1 Inflammatory Signaling.; Authors - Hong Xiang
mouse,human	WB,IHC	Am J Pathol; Cross Talk between ST2 and Transforming Growth Factor-β Receptor Signaling Promotes Renal Fibrosis; Authors - Xingxing Zhu
human	WB	EBioMedicine; Free fatty acid receptor 4 deletion attenuates colitis by modulating Treg Cells via ZBED6-IL33 pathway.; Authors - Shenglong Zhu
mouse,human	IHC	Int Immunopharmacol; Hypoxia induces the production of epithelial-derived cytokines in eosinophilic chronic rhinosinusitis with nasal polyps; Authors - Meiping Zhang

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