Anticorps Recombinant de lapin anti-IL3RA/CD123

IL3RA/CD123 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240042E5

N° de cat : 83243-3-RR

Synonymes

IL3RA, 240042E5, CD123, hIL 3Ra, IL 3 receptor subunit alpha

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Galerie de données de validation

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  WB analysis of HL-60 using 83243-3-RR

  HL-60 cells were subjected to SDS PAGE followed by western blot with 83243-3-RR (IL3RA/CD123 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83243-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83243-3-RR against Human IL3RA/CD123 were performed. The affinity constant is 4.88 nM.

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Applications testées

• Résultats positifs en WB: cellules HL-60,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83243-3-RR cible IL3RA/CD123 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IL3RA/CD123 Protéine recombinante Ag4452
• Nom complet: interleukin 3 receptor, alpha (low affinity)
• Masse moléculaire calculée: 378 aa, 43 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC035407
• Symbole du gène: CD123
• Identification du gène (NCBI): 3563
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-3 receptor subunit alpha (IL3RA), also known as CD123, belongs to the cytokine receptor family. It is a glycoprotein containing an extracellular domain, involving a predicted Ig-like domain, two FnIII domains, a transmembrane domain and an intracellular domain (PMID: 24513123). IL3RA is expressed on hematopoietic progenitor cells, plasmacytoid dendritic cells, basophils, monocytes, some B cells, and in a variety of hematologic malignancies (PMID: 10527461; 34855461). IL-3 initially binds to IL3RA and subsequently recruits the beta chain (CD131) to form the high-affinity receptor, resulting in the activation of a series of signaling pathways including the JAK/STAT, Ras-MAPK, and phosphatidylinositol 3-kinase pathways (PMID: 29296749). IL-3R signaling regulates the proliferation, survival, and differentiation of hematopoietic cells.

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