Anticorps Recombinant de lapin anti-IL3RA/CD123

IL3RA/CD123 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Biotin

CloneNo.

241268E3

N° de cat : Biotin-98107

Synonymes

IL3RA, IL 3R subunit alpha, IL 3R alpha, IL 3 receptor subunit alpha, hIL 3Ra

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Galerie de données de validation

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  FC experiment of human monocyte-derived immature dendritic cells using Biotin-98107

  1x10^6 human monocyte-derived immature dendritic cells were surface stained with 0.25 ug Biotin Plus Anti-Human IL3RA/CD123 Rabbit Recombinant Antibody (Biotin-98107, Clone: 241268E3) and CoraLite®488-conjugated streptavidin, or unstained. Cells were co-stained with CoraLite® Plus 647 Anti-Human CD45RO (UCHL1) (CL647-65150, Clone: UCHL1). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human monocyte-derived immature dendritic cells using Biotin-98107

  1x10^6 human monocyte-derived immature dendritic cells were surface stained with 0.25 ug Biotin Plus Anti-Human IL3RA/CD123 Rabbit Recombinant Antibody (Biotin-98107, Clone: 241268E3) (red) and CoraLite®488-conjugated streptavidin, or unstained (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: human monocyte-derived immature dendritic cells

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

Biotin-98107 cible IL3RA/CD123 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 3 receptor, alpha (low affinity)
• Masse moléculaire calculée: 378 aa, 43 kDa
• Numéro d’acquisition GenBank: BC035407
• Symbole du gène: CD123
• Identification du gène (NCBI): 3563
• Conjugaison: Biotin
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Interleukin-3 receptor subunit alpha (IL3RA), also known as CD123, belongs to the cytokine receptor family. It is a glycoprotein containing an extracellular domain, involving a predicted Ig-like domain, two FnIII domains, a transmembrane domain and an intracellular domain (PMID: 24513123). IL3RA is expressed on hematopoietic progenitor cells, plasmacytoid dendritic cells, basophils, monocytes, some B cells, and in a variety of hematologic malignancies (PMID: 10527461; 34855461). IL-3 initially binds to IL3RA and subsequently recruits the beta chain (CD131) to form the high-affinity receptor, resulting in the activation of a series of signaling pathways including the JAK/STAT, Ras-MAPK, and phosphatidylinositol 3-kinase pathways (PMID: 29296749). IL-3R signaling regulates the proliferation, survival, and differentiation of hematopoietic cells.

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