Anticorps Recombinant de lapin anti-IL3RA/CD123

IL3RA/CD123 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

241268E3

N° de cat : CL647-98107

Synonymes

IL3RA, IL 3R subunit alpha, IL 3R alpha, IL 3 receptor subunit alpha, hIL 3Ra

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Galerie de données de validation

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  FC experiment of human PBMCs using CL647-98107

  1x10^6 FC PBMCs were surface stained with FITC Plus Anti-Human CD45RO and 5 ul CoraLite® Plus 647 Anti-Human IL3RA/CD123 Rabbit Recombinant Antibody (CL647-98107, Clone:241268E3), or CoraLite® Plus 647 Rabbit IgG Isotype Control Recombinant Antibody (CL647-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL647-98107

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human IL3RA/CD123 Rabbit Recombinant Antibody (CL647-98107, Clone:241268E3)(red), or CoraLite® Plus 647 Rabbit IgG Isotype Control Recombinant Antibody (CL647-98136, Clone: 240953C9)(blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-98107 cible IL3RA/CD123 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 3 receptor, alpha (low affinity)
• Masse moléculaire calculée: 378 aa, 43 kDa
• Numéro d’acquisition GenBank: BC035407
• Symbole du gène: CD123
• Identification du gène (NCBI): 3563
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Interleukin-3 receptor subunit alpha (IL3RA), also known as CD123, belongs to the cytokine receptor family. It is a glycoprotein containing an extracellular domain, involving a predicted Ig-like domain, two FnIII domains, a transmembrane domain and an intracellular domain (PMID: 24513123). IL3RA is expressed on hematopoietic progenitor cells, plasmacytoid dendritic cells, basophils, monocytes, some B cells, and in a variety of hematologic malignancies (PMID: 10527461; 34855461). IL-3 initially binds to IL3RA and subsequently recruits the beta chain (CD131) to form the high-affinity receptor, resulting in the activation of a series of signaling pathways including the JAK/STAT, Ras-MAPK, and phosphatidylinositol 3-kinase pathways (PMID: 29296749). IL-3R signaling regulates the proliferation, survival, and differentiation of hematopoietic cells.

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