Anticorps Recombinant de lapin anti-IL-4

IL-4 Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

240794H7

N° de cat : 83894-5-PBS

Synonymes

IL4, B-cell stimulatory factor 1, BSF-1, IL 4, interleukin 4

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Galerie de données de validation

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  Sandwich ELISA standard curve of MP00829-3

  Sandwich ELISA standard curve of MP00829-3, Human IL-4 Recombinant Matched Antibody Pair - PBS only. 83894-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0104. 83894-5-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL

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  Sample test of MP00829-3

  Serum of sixteen individual healthy human donors was measured. The human IL-4 concentration of detected samples was determined to be 7.97 pg/mL with a range of 2.25 - 19.49 pg/mL.

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  Sample test of MP00829-3

  Human peripheral blood mononuclear cells (0.5 x 10^6 cells/mL) were cultured in RPMI supplemented with 10% fetal bovine serum, 5 μg/mL PHA, and 10 ng/mL recombinant human IL-2. After 7 days of culture, cells were changed into DMEM with 10% fetal bovine serum, 10 ng/mL PMA, 1 μg/mL Ca2+ ionophore, and 5 μg/mL PHA. Cell culture supernate was collected after 1d of stimulation and measured 292.5 pg/mL in stimulated PBMC supernatant, 11.2 pg/mL in unstimulated PBMC supernatant.

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  Cytometric bead array standard curve of MP00829-3

  Cytometric bead array standard curve of MP00829-3, IL-4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83894-2-PBS. Detection antibody: 83894-5-PBS. Standard: Eg0104. Range: 0.625-40 ng/mL.

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  Affinity and Kinetic Characterization of 83894-5-PBS

  Biolayer interferometry (BLl) kinetic assays of 83894-5-PBS against Human IL-4 were performed. The affinity constant is 1.11 nM.

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Informations sur le produit

83894-5-PBS cible IL-4 dans les applications de Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IL-4 Protéine recombinante Eg0104
• Nom complet: interleukin 4
• Masse moléculaire calculée: 153 aa, 17 kDa
• Numéro d’acquisition GenBank: BC070123
• Symbole du gène: IL-4
• Identification du gène (NCBI): 3565
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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