Anticorps Monoclonal anti-IL-4RA/CD124

IL-4RA/CD124 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

1E4C11

N° de cat : 67051-1-Ig

Synonymes

IL4R, 1E4C11, CD124, IL 4 binding protein, IL 4 receptor subunit alpha

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Galerie de données de validation

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  WB analysis using 67051-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67051-1-Ig (IL-4RA/CD124 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of MG-63 using 67051-1-Ig

  MG-63 cells were subjected to SDS PAGE followed by western blot with 67051-1-Ig (IL-4RA/CD124 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67051-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67051-1-Ig (IL-4RA/CD124 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules Daudi, cellules HUVEC, cellules Jurkat, cellules Raji, cellules Ramos

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67051-1-Ig cible IL-4RA/CD124 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IL-4RA/CD124 Protéine recombinante Ag28411
• Nom complet: interleukin 4 receptor
• Masse moléculaire calculée: 90 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: NM_000418
• Symbole du gène: IL-4R
• Identification du gène (NCBI): 3566
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IL-4 receptor alpha chain (IL-4RA), also known as CD124, is a transmembrane glycoprotein expressed on a wide variety of hematopoietic and non-hematopoietic cells. It is a receptor for both IL-4 and IL-13. IL-4RA can complex with the common gamma chain (IL-2R gamma or CD132) and utilizes JAK1 and JAK2 for signal transduction, while complexes of IL-4RA with IL-13RA1 subunit mediate signals via JAK2 and Tyk2. The IL-4 response is involved in promoting Th2 differentiation. The IL-4/IL-13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation.

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