Anticorps Polyclonal de lapin anti-IL-5RA/CD125

IL-5RA/CD125 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 12655-1-AP

Synonymes

IL5RA, CD125, HSIL5R3, IL 5 receptor subunit alpha, IL 5R alpha

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Galerie de données de validation

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  WB analysis of human peripheral blood platelets using 12655-1-AP

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 12655-1-AP (IL-5RA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12655-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12655-1-AP (IL5RA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: plaquettes de sang périphérique humain, cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

12655-1-AP cible IL-5RA/CD125 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IL-5RA/CD125 Protéine recombinante Ag3358
• Nom complet: interleukin 5 receptor, alpha
• Masse moléculaire calculée: 48 kDa, 38 kDa
• Poids moléculaire observé: 70-75 kDa, 43 kDa
• Numéro d’acquisition GenBank: BC027599
• Symbole du gène: IL-5RA/CD125
• Identification du gène (NCBI): 3568
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-5 receptor subunit alpha (IL-5RA), also known as CD125, is a type I transmembrane protein that belongs to the type I cytokine receptor family. IL-5RA is a critical component of the high-affinity IL-5 receptor complex, IL-5R, which consists of the alpha chain (IL-5RA) that specifically binds to IL-5 and the common beta chain (CD131) shared with receptors for IL-3 and GM-CSF (PMID: 1833065; 7872157). IL-5/IL-5RA signaling axis is crucial for the differentiation, proliferation, recruitment, and activation of eosinophils, and is also involved in pathological eosinophilia (PMID: 34638583).

Publications

Species	Application	Title
human		J Allergy Clin Immunol; Mepolizumab does not alter levels of eosinophils, T cells, and mast cells in the duodenal mucosa in eosinophilic esophagitis; Authors - Sébastien Conus
human		Front Immunol; NET Formation in Bullous Pemphigoid Patients With Relapse Is Modulated by IL-17 and IL-23 Interplay.; Authors - Delphine Giusti

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