Anticorps Polyclonal de lapin anti-CD126/IL-6R alpha

• Phare
• Validé par KD/KO

CD126/IL-6R alpha Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(47)

Avis (1)

N° de cat : 23457-1-AP

Synonymes

CD126, IL-6R alpha, gp80, IL 6 receptor subunit alpha, IL 6R 1

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Galerie de données de validation

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  WB analysis using 23457-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23457-1-AP (CD126/IL-6R alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 23457-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23457-1-AP (IL-6R alpha antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse spleen using 23457-1-AP

  IP result of anti-CD126/IL-6R alpha (IP:23457-1-AP, 4ug; Detection:23457-1-AP 1:3000) with mouse spleen tissue lysate 2480 ug.

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  IHC staining of mouse spleen using 23457-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 23457-1-AP (CD126/IL-6R alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 23457-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 23457-1-AP (CD126/IL-6R alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu splénique de souris, cellules Jurkat, tissu splénique de rat
• Résultats positifs en IP: tissu splénique de souris,
• Résultats positifs en IHC: tissu splénique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 33 publications below
• IHC: See 8 publications below
• IF: See 9 publications below
• ELISA: See 1 publications below

Informations sur le produit

23457-1-AP cible CD126/IL-6R alpha dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD126/IL-6R alpha Protéine recombinante Ag18263
• Nom complet: interleukin 6 receptor
• Masse moléculaire calculée: 468 aa, 52 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC132684
• Symbole du gène: IL-6R alpha
• Identification du gène (NCBI): 3570
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R alpha, the complex of IL-6 and IL-6R alpha associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while expression of IL-6R alpha is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892). Soluble form of IL-6R alpha has been found in human serum and urine (PMID: 2529343; 1545125).

Publications

Species	Application	Title
rat	IHC	Signal Transduct Target Ther; Metabolic reprogramming of proinflammatory macrophages by target delivered roburic acid effectively ameliorates rheumatoid arthritis symptoms; Authors - Na Jia
mouse	WB	Cancer Discov; Loss of Optineurin Drives Cancer Immune Evasion via Palmitoylation-Dependent IFNGR1 Lysosomal Sorting and Degradation.; Authors - Wan Du
human	IF	J Med Virol; Molecular and immune signatures, and pathological trajectories of fatal COVID-19 lungs defined by in situ spatial single-cell transcriptome analysis; Authors - Arun Das
mouse,human	IF	Acta Pharmacol Sin; Exosomes derived from induced cardiopulmonary progenitor cells alleviate acute lung injury in mice; Authors - Luo-Xing Xia
rat	WB	Int J Biol Macromol; A dual gene-activated dermal scaffolds loaded with nanocomposite particles expressing of VEGF and aFGF: Promoting wound healing by early vascularization; Authors - Jialiang Wang
mouse	WB	Allergol Int; Prevention of IL-6 signaling ameliorates toluene diisocyanate-induced steroid-resistant asthma.; Authors - Shuyu Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jianhua (Verified Customer) (04-02-2025)

works very well for western blot

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human T-cells

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