Anticorps Polyclonal de lapin anti-IL-6R alpha

• Phare
• Validé par KD/KO

IL-6R alpha Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

26

N° de cat : 23457-1-AP

Synonymes

CD126, gp80, IL 6 receptor subunit alpha, IL 6R 1, IL 6R alpha, IL 6R subunit alpha, IL 6RA, IL-6 R alpha, IL-6R, IL-6R alpha, IL6RA, interleukin 6 receptor, Membrane glycoprotein 80

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Galerie de données de validation

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  WB analysis of Jurkat using 23457-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23457-1-AP (IL-6R alpha antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 23457-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 23457-1-AP (IL-6R alpha antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 23457-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23457-1-AP (IL-6R alpha antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse spleen using 23457-1-AP

  IP Result of anti-IL-6R alpha (IP:23457-1-AP, 4ug; Detection:23457-1-AP 1:500) with mouse spleen tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, tissu splénique de rat, tissu splénique de souris
• Résultats positifs en IP: tissu splénique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 21 publications below
• IHC: See 3 publications below
• IF: See 6 publications below

Informations sur le produit

23457-1-AP cible IL-6R alpha dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IL-6R alpha Protéine recombinante Ag18263
• Nom complet: interleukin 6 receptor
• Masse moléculaire calculée: 468 aa, 52 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC132684
• Symbole du gène: IL6R
• Identification du gène (NCBI): 3570
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R alpha, the complex of IL-6 and IL-6R alpha associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while expression of IL-6R alpha is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892). Soluble form of IL-6R alpha has been found in human serum and urine (PMID: 2529343; 1545125).

Publications

Species	Application	Title
rat	IHC	Signal Transduct Target Ther; Metabolic reprogramming of proinflammatory macrophages by target delivered roburic acid effectively ameliorates rheumatoid arthritis symptoms; Authors - Na Jia
mouse	WB	Cancer Discov; Loss of Optineurin Drives Cancer Immune Evasion via Palmitoylation-Dependent IFNGR1 Lysosomal Sorting and Degradation.; Authors - Wan Du
human	IF	J Med Virol; Molecular and immune signatures, and pathological trajectories of fatal COVID-19 lungs defined by in situ spatial single-cell transcriptome analysis; Authors - Arun Das
mouse	WB	Allergol Int; Prevention of IL-6 signaling ameliorates toluene diisocyanate-induced steroid-resistant asthma.; Authors - Shuyu Chen
human	WB	Pharmacol Res; Discovery of a novel megakaryopoiesis enhancer, ingenol, promoting thrombopoiesis through PI3K-Akt signaling independent of thrombopoietin.; Authors - Long Wang
human	WB,IF	Cell Prolif; Increased SPON1 promotes pancreatic ductal adenocarcinoma progression by enhancing IL-6 trans-signalling.; Authors - Yanmiao Huo

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