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Anticorps Recombinant de lapin anti-CD126/IL-6R alpha

CD126/IL-6R alpha Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240734D1

N° de cat : 83688-4-PBS

Synonymes

CD126, IL-6R alpha, 240734D1, IL 6R alpha, IL 6R 1

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of RAW 264.7 cells using CD126/IL-6R alpha Recombinant antibody (83688-4-RR)

    WB analysis of RAW 264.7 using 83688-4-RR (same clone as 83688-4-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 83688-4-RR (CD126/IL-6R alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83688-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83688-4-RR

    Affinity and Kinetic Characterization of 83688-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83688-4-RR against Human CD126/IL-6R alpha were performed. The affinity constant is 0.80 nM.

Informations sur le produit

83688-4-PBS cible CD126/IL-6R alpha dans les applications de WB, ELISA et montre une réactivité avec des échantillons souris

Réactivité souris
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène Protéine recombinante
Nom complet interleukin 6 receptor
Masse moléculaire calculée 468 aa, 52 kDa
Poids moléculaire observé65 kDA
Numéro d’acquisition GenBankBC132684
Symbole du gène IL-6R alpha
Identification du gène (NCBI) 3570
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Protein A purfication
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R, the complex of IL-6 and IL-6R associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while IL-6R is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892).

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