Anticorps Polyclonal de lapin anti-CD127/IL-7R

CD127/IL-7R Polyclonal Antibody for FC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, FC, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 17626-1-AP

Synonymes

CD127, CDW127, IL 7 receptor subunit alpha, IL 7R alpha, IL 7R subunit alpha, IL 7RA, IL7R, IL-7R, IL7RA, ILRA, interleukin 7 receptor

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Galerie de données de validation

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  WB analysis of mouse thymus using 17626-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17626-1-AP (CD127/IL7R antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 17626-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17626-1-AP (CD127/IL7R antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 17626-1-AP

  IP Result of anti-CD127/IL7R (IP:17626-1-AP, 4ug; Detection:17626-1-AP 1:1000) with K-562 cells lysate 1200ug.

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  FC experiment of K-562 using 17626-1-AP

  1X10^6 K-562 cells were stained with 0.2ug CD127/IL7R antibody (17626-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: tissu de thymus de souris, cellules K-562
• Résultats positifs en IP: cellules K-562
• Résultats positifs en cytométrie: cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3600
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

17626-1-AP cible CD127/IL-7R dans les applications de WB, IP, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD127/IL-7R Protéine recombinante Ag11844
• Nom complet: interleukin 7 receptor
• Masse moléculaire calculée: 459 aa, 52 kDa
• Poids moléculaire observé: 56-58 kDa
• Numéro d’acquisition GenBank: BC067537
• Symbole du gène: IL7R
• Identification du gène (NCBI): 3575
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD127, also known as IL-7R subunit alpha (IL-7RA), is a type I membrane glycoprotein expressed on thymocytes, B cell precursors, most T cells, and some lymphoid and myeloid cells. IL-7R is a heterodimer composed of CD127 and the common-γ chain receptor (CD132). IL-7R plays critical roles in lymphocyte development and homeostasis. CD127 can also act as a receptor for thymic stromal lymphopoietin (TSLP).

Publications

Species	Application	Title
human,mouse	WB	J Mammary Gland Biol Neoplasia; TGFß1 Stimulates Lymphatic Endothelial Cells to Produce IL7 and IL15, Which Act as Chemotactic Factors for Breast Cancer Cells with Mesenchymal Properties; Authors - Nikolina Giotopoulou
human	WB	Mol Med Rep; TET2 is involved in DNA hydroxymethylation, cell proliferation and inflammatory response in keratinocytes.; Authors - Xinxin Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Bastien (Verified Customer) (08-19-2020)

Western blotting of protein from B cells of mice spleen. Protein extract was loaded on 8 % polyacrylamide gel and then transfert on nitrocellulose membrane. Nitrocellulose membrane was blocked with 5% BSA during 1h. Membrane was then incubated overnight at 4°C with 1/500 IL7Ra antibody (17626-1-AP). Finally, the membrane was incubated with anti-rabbit-HRP (clean blot IPdetection reagent from thermofisher)

• Applications: Western Blot
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: B cells

CD127/IL-7R Antibody Western Blot validation (1/500 dilution) in B cells (Cat no:17626-1-AP)

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