Anticorps Polyclonal de lapin anti-IL-7Ra/CD127
IL-7Ra/CD127 Polyclonal Antibody for WB, IF-P, IP, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, souris
Applications
WB, IF-P, IP, ELISA
Conjugaison
Non conjugué
N° de cat : 17626-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | tissu de thymus de souris, cellules K-562 | 
| Résultats positifs en IP | cellules K-562 | 
| Résultats positifs en IF-P | tissu splénique de souris, | 
Dilution recommandée
| Application | Dilution | 
|---|---|
| Western Blot (WB) | WB : 1:500-1:3600 | 
| Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate | 
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 4 publications below | 
Informations sur le produit
17626-1-AP cible IL-7Ra/CD127 dans les applications de WB, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, souris
| Réactivité | Humain, souris | 
| Réactivité citée | Humain, souris | 
| Hôte / Isotype | Lapin / IgG | 
| Clonalité | Polyclonal | 
| Type | Anticorps | 
| Immunogène | IL-7Ra/CD127 Protéine recombinante Ag11844 | 
| Nom complet | interleukin 7 receptor | 
| Masse moléculaire calculée | 459 aa, 52 kDa | 
| Poids moléculaire observé | 56-58 kDa | 
| Numéro d’acquisition GenBank | BC067537 | 
| Symbole du gène | CD127 | 
| Identification du gène (NCBI) | 3575 | 
| Conjugaison | Non conjugué | 
| Forme | Liquide | 
| Méthode de purification | Purification par affinité contre l'antigène | 
| Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol | 
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. | 
Informations générales
CD127, also known as IL-7R subunit alpha (IL-7RA), is a type I membrane glycoprotein expressed on thymocytes, B cell precursors, most T cells, and some lymphoid and myeloid cells. IL-7R is a heterodimer composed of CD127 and the common-γ chain receptor (CD132). IL-7R plays critical roles in lymphocyte development and homeostasis. CD127 can also act as a receptor for thymic stromal lymphopoietin (TSLP).
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-7Ra/CD127 antibody 17626-1-AP | Download protocol | 
| IF protocol for IL-7Ra/CD127 antibody 17626-1-AP | Download protocol | 
| IP protocol for IL-7Ra/CD127 antibody 17626-1-AP | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 
Publications
| Species | Application | Title | 
|---|---|---|
| J Mammary Gland Biol Neoplasia TGFß1 Stimulates Lymphatic Endothelial Cells to Produce IL7 and IL15, Which Act as Chemotactic Factors for Breast Cancer Cells with Mesenchymal Properties | ||
| Mol Med Rep TET2 is involved in DNA hydroxymethylation, cell proliferation and inflammatory response in keratinocytes. | ||
| Mol Cancer Targeting BATF2-RGS2 axis reduces T-cell exhaustion and restores anti-tumor immunity | ||
| Clin Cosmet Investig Dermatol Identification of a Gene Expression Signature to Predict the Risk of Abdominal Aortic Aneurysm in Psoriasis Patients | 
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
| FH Bastien (Verified Customer) (08-19-2020) | Western blotting of protein from B cells of mice spleen. Protein extract was loaded on 8 % polyacrylamide gel and then transfert on nitrocellulose membrane. Nitrocellulose membrane was blocked with 5% BSA during 1h. Membrane was then incubated overnight at 4°C with 1/500 IL7Ra antibody (17626-1-AP). Finally, the membrane was incubated with anti-rabbit-HRP (clean blot IPdetection reagent from thermofisher) 
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