Anticorps Polyclonal de lapin anti-IL-7Ra/CD127

IL-7Ra/CD127 Polyclonal Antibody for WB, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 17626-1-AP

Synonymes

CD127, IL7 receptor subunit alpha, IL 7RA, IL 7R subunit alpha, IL 7R alpha



Applications testées

Résultats positifs en WBtissu de thymus de souris, cellules K-562
Résultats positifs en IPcellules K-562
Résultats positifs en IF-Ptissu splénique de souris,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3600
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

WBSee 4 publications below

Informations sur le produit

17626-1-AP cible IL-7Ra/CD127 dans les applications de WB, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Réactivité citéeHumain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène IL-7Ra/CD127 Protéine recombinante Ag11844
Nom complet interleukin 7 receptor
Masse moléculaire calculée 459 aa, 52 kDa
Poids moléculaire observé 56-58 kDa
Numéro d’acquisition GenBankBC067537
Symbole du gène CD127
Identification du gène (NCBI) 3575
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD127, also known as IL-7R subunit alpha (IL-7RA), is a type I membrane glycoprotein expressed on thymocytes, B cell precursors, most T cells, and some lymphoid and myeloid cells. IL-7R is a heterodimer composed of CD127 and the common-γ chain receptor (CD132). IL-7R plays critical roles in lymphocyte development and homeostasis. CD127 can also act as a receptor for thymic stromal lymphopoietin (TSLP).

Protocole

Product Specific Protocols
WB protocol for IL-7Ra/CD127 antibody 17626-1-APDownload protocol
IF protocol for IL-7Ra/CD127 antibody 17626-1-APDownload protocol
IP protocol for IL-7Ra/CD127 antibody 17626-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

J Mammary Gland Biol Neoplasia

TGFß1 Stimulates Lymphatic Endothelial Cells to Produce IL7 and IL15, Which Act as Chemotactic Factors for Breast Cancer Cells with Mesenchymal Properties

Authors - Nikolina Giotopoulou
humanWB

Mol Med Rep

TET2 is involved in DNA hydroxymethylation, cell proliferation and inflammatory response in keratinocytes.

Authors - Xinxin Liu
mouseWB

Mol Cancer

Targeting BATF2-RGS2 axis reduces T-cell exhaustion and restores anti-tumor immunity

Authors - Xuyu Gu
humanWB

Clin Cosmet Investig Dermatol

Identification of a Gene Expression Signature to Predict the Risk of Abdominal Aortic Aneurysm in Psoriasis Patients

Authors - Xintong Lyu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Bastien (Verified Customer) (08-19-2020)

Western blotting of protein from B cells of mice spleen. Protein extract was loaded on 8 % polyacrylamide gel and then transfert on nitrocellulose membrane. Nitrocellulose membrane was blocked with 5% BSA during 1h. Membrane was then incubated overnight at 4°C with 1/500 IL7Ra antibody (17626-1-AP). Finally, the membrane was incubated with anti-rabbit-HRP (clean blot IPdetection reagent from thermofisher)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/500
  • Cell Tissue Type: B cells
IL-7Ra/CD127 Antibody Western Blot validation (1/500 dilution) in B cells (Cat no:17626-1-AP)
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