Anticorps Monoclonal anti-IL-9

IL-9 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(6)

CloneNo.

1F3A11

N° de cat : 66144-1-Ig

Synonymes

IL9, 1F3A11, IL 9, interleukin 9, interleukin9

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Galerie de données de validation

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  WB analysis of Recombinant protein using 66144-1-Ig

  Recombinant protein were subjected to SDS PAGE followed by western blot with 66144-1-Ig (IL9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 66144-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66144-1-Ig (IL-9 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66144-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66144-1-Ig (IL-9 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 66144-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-9 antibody (66144-1-Ig, Clone: 1F3A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: Protéine recombinante, protéine protéine recombinante
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu d'amygdalite humain,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

66144-1-Ig cible IL-9 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IL-9 Protéine recombinante Ag21435
• Nom complet: interleukin 9
• Masse moléculaire calculée: 144 aa, 16 kDa
• Poids moléculaire observé: 19 kDa
• Numéro d’acquisition GenBank: BC066284
• Symbole du gène: IL9
• Identification du gène (NCBI): 3578
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin 9 (IL-9) is a γc-family cytokine that is highly produced by T-helper 9 (Th9) cells. IL-9 could promote the survival and activation of various cellular targets, including mast cells, B cells, T cells, and structural cells. Its expression is considered a hallmark of Th2-lineage cells. Primarily studied in Th2-type immunity, IL-9 was shown to be involved in asthma, allergy, and host defense against helminth infections. IL-9 also stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes.(PMID: 38864109; PMID: 29038115; PMID: 9505195; PMID: 29703631)

Publications

Species	Application	Title
human	WB	FEBS Open Bio; Bach2 overexpression represses Th9 cell differentiation by suppressing IRF4 expression in systemic lupus erythematosus.; Authors - Yujun Sheng
mouse		Cell Rep Med; Deficiency of the CD155-CD96 immune checkpoint controls IL-9 production in giant cell arteritis; Authors - Shozo Ohtsuki
human	IHC	Clin Cosmet Investig Dermatol; Higher IL-9 Level is Associated with Psoriasis Vulgaris Complicated by Metabolic Syndrome; Authors - Liang Yan
mouse,human	IHC,IF	Biomolecules; Association of IL-9 Cytokines with Hepatic Injury in Echinococcus granulosus Infection; Authors - Tanfang Zhou
human	IHC	J Trop Med; A Series of 35 Cutaneous Infections Caused by Mycobacterium marinum in Han Chinese Population; Authors - Wenjie Chen
human	IF	Cancer Immunol Immunother; Impact of innate lymphoid cell type 2 in chronic lymphocytic leukemia on the function of treg and CD8+ T cells through IL-9; Authors - Ruixue Yang

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