Anticorps Polyclonal de lapin anti-ILK

ILK Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 24926-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse heart using 24926-1-AP (same clone as 24926-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 24926-1-AP (ILK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24926-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 24926-1-AP (same clone as 24926-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24926-1-AP (ILK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24926-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 24926-1-AP (same clone as 24926-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24926-1-AP (ILK Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24926-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 24926-1-AP (same clone as 24926-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24926-1-AP (ILK Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24926-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 24926-1-AP (same clone as 24926-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 24926-1-AP (ILK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24926-1-PBS in a different storage buffer formulation.

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Informations sur le produit

24926-1-PBS cible ILK dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ILK Protéine recombinante Ag21494
• Nom complet: integrin-linked kinase
• Masse moléculaire calculée: 451 aa, 59 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC001554
• Symbole du gène: ILK
• Identification du gène (NCBI): 3611
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ILK(Integrin-linked protein kinase) is also named as p59, PAT-4, DKFZp686F1765, ILK-1, ILK-2, ILK1, ILK2, p59ILK and belongs to the TKL Ser/Thr protein kinase family. This protein localizes to multiple sites, including the cytoplasm, and imports into the nucleus through sequences in its N-terminus, via active transport mechanisms that involve nuclear pore complexes(PMID: 18635968). ILK performs crucial roles in the control of intestinal cell and crypt-villus axis homeostasis-especially with regard to basement membrane fibronectin deposition-as well as cell proliferation, spreading, and migration(PMID:19885839).

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