Anticorps Monoclonal anti-ILK

ILK Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

1E10E4

N° de cat : 67724-1-Ig

Synonymes

DKFZp686F1765, ILK, ILK 1, ILK 2, ILK1, ILK2, integrin linked kinase, Integrin linked protein kinase, P59, p59ILK

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Galerie de données de validation

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  WB analysis using 67724-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67724-1-Ig (ILK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of human peripheral blood platelets using 67724-1-Ig

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67724-1-Ig (ILK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 67724-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67724-1-Ig (ILK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig colon using 67724-1-Ig

  pig colon tissue were subjected to SDS PAGE followed by western blot with 67724-1-Ig (ILK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 67724-1-Ig

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 67724-1-Ig (ILK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat colon using 67724-1-Ig

  rat colon tissue were subjected to SDS PAGE followed by western blot with 67724-1-Ig (ILK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules U2OS, cellules HEK-293, cellules HeLa, cellules HepG2, cellules K-562, plaquettes de sang périphérique humain, tissu de côlon de porc, tissu de côlon de rat, tissu de côlon de souris, tissu placentaire humain

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67724-1-Ig cible ILK dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ILK Protéine recombinante Ag21289
• Nom complet: integrin-linked kinase
• Masse moléculaire calculée: 451 aa, 59 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC001554
• Symbole du gène: ILK
• Identification du gène (NCBI): 3611
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ILK(Integrin-linked protein kinase) is also named as p59, PAT-4, DKFZp686F1765, ILK-1, ILK-2, ILK1, ILK2, p59ILK and belongs to the TKL Ser/Thr protein kinase family. This protein localizes to multiple sites, including the cytoplasm, and imports into the nucleus through sequences in its N-terminus, via active transport mechanisms that involve nuclear pore complexes(PMID: 18635968). ILK performs crucial roles in the control of intestinal cell and crypt-villus axis homeostasis-especially with regard to basement membrane fibronectin deposition-as well as cell proliferation, spreading, and migration(PMID:19885839).