Anticorps Monoclonal anti-ILK

ILK Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E10E4

N° de cat : 67724-1-PBS

Synonymes

ILK 2, ILK 1, EC:2.7.11.1, Beta-integrin-linked kinase, 59 kDa serine/threonine-protein kinase

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Galerie de données de validation

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  WB analysis using 67724-1-Ig (same clone as 67724-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67724-1-Ig (ILK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67724-1-PBS in a different storage buffer formulation.

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  WB analysis of human peripheral blood platelets using 67724-1-Ig (same clone as 67724-1-PBS)

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67724-1-Ig (ILK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67724-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 67724-1-Ig (same clone as 67724-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67724-1-Ig (ILK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67724-1-PBS in a different storage buffer formulation.

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  WB analysis of pig colon using 67724-1-Ig (same clone as 67724-1-PBS)

  pig colon tissue were subjected to SDS PAGE followed by western blot with 67724-1-Ig (ILK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67724-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse colon using 67724-1-Ig (same clone as 67724-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 67724-1-Ig (ILK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67724-1-PBS in a different storage buffer formulation.

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  WB analysis of rat colon using 67724-1-Ig (same clone as 67724-1-PBS)

  rat colon tissue were subjected to SDS PAGE followed by western blot with 67724-1-Ig (ILK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67724-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 67724-1-Ig (same clone as 67724-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 67724-1-Ig (ILK antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67724-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67724-1-PBS cible ILK dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ILK Protéine recombinante Ag21289
• Nom complet: integrin-linked kinase
• Masse moléculaire calculée: 451 aa, 59 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC001554
• Symbole du gène: ILK
• Identification du gène (NCBI): 3611
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ILK(Integrin-linked protein kinase) is also named as p59, PAT-4, DKFZp686F1765, ILK-1, ILK-2, ILK1, ILK2, p59ILK and belongs to the TKL Ser/Thr protein kinase family. This protein localizes to multiple sites, including the cytoplasm, and imports into the nucleus through sequences in its N-terminus, via active transport mechanisms that involve nuclear pore complexes(PMID: 18635968). ILK performs crucial roles in the control of intestinal cell and crypt-villus axis homeostasis-especially with regard to basement membrane fibronectin deposition-as well as cell proliferation, spreading, and migration(PMID:19885839).

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