Anticorps Recombinant de lapin anti-ILK

ILK Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

242982A5

N° de cat : 85428-5-RR

Synonymes

59 kDa serine/threonine-protein kinase, Beta-integrin-linked kinase, EC:2.7.11.1, ILK 1, ILK 2

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Galerie de données de validation

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  WB analysis using 85428-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85428-5-RR (ILK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig colon using 85428-5-RR

  pig colon tissue were subjected to SDS PAGE followed by western blot with 85428-5-RR (ILK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 85428-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ILK antibody (85428-5-RR, Clone: 242982A5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 85428-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85428-5-RR against Human ILK were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HEK-293, cellules HeLa, tissu cérébral de souris, tissu de côlon de porc
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85428-5-RR cible ILK dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ILK Protéine recombinante Ag3570
• Nom complet: integrin-linked kinase
• Masse moléculaire calculée: 451 aa, 59 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC001554
• Symbole du gène: ILK
• Identification du gène (NCBI): 3611
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ILK(Integrin-linked protein kinase) is also named as p59, PAT-4, DKFZp686F1765, ILK-1, ILK-2, ILK1, ILK2, p59ILK and belongs to the TKL Ser/Thr protein kinase family. This protein localizes to multiple sites, including the cytoplasm, and imports into the nucleus through sequences in its N-terminus, via active transport mechanisms that involve nuclear pore complexes(PMID: 18635968). ILK performs crucial roles in the control of intestinal cell and crypt-villus axis homeostasis-especially with regard to basement membrane fibronectin deposition-as well as cell proliferation, spreading, and migration(PMID:19885839).

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