Anticorps Polyclonal de lapin anti-IMP3

• Phare
• Validé par KD/KO

IMP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12750-1-PBS

Synonymes

MRPS4, IMP 3, C15orf12, BRMS2

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Galerie de données de validation

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  WB analysis of HeLa using 12750-1-AP (same clone as 12750-1-PBS)

  WB result of IMP3 antibody (12750-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IMP3 transfected HeLa cells. This data was developed using the same antibody clone with 12750-1-PBS in a different storage buffer formulation.

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  WB analysis using 12750-1-AP (same clone as 12750-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12750-1-AP (IMP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12750-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 12750-1-AP (same clone as 12750-1-PBS)

  IP result of anti-IMP3 (IP:12750-1-AP, 3ug; Detection:12750-1-AP 1:500) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 12750-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 12750-1-AP (same clone as 12750-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12750-1-AP (IMP3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12750-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 12750-1-AP (same clone as 12750-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IMP3 antibody (12750-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12750-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12750-1-PBS cible IMP3 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IMP3 Protéine recombinante Ag3477
• Nom complet: IMP3, U3 small nucleolar ribonucleoprotein, homolog (yeast)
• Masse moléculaire calculée: 184 aa, 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC020557
• Symbole du gène: IMP3
• Identification du gène (NCBI): 55272
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IMP U3 small nucleolar ribonucleoprotein 3 (IMP3, BRMS2), is a component of U3 small nucleolar ribonucleoprotein complex (U3 snoRNP), has been identified as a participant in pre-rRNA processing for nearly twenty years.

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