Anticorps Recombinant de lapin anti-IMPDH1

IMPDH1 Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242329D8

N° de cat : 84913-1-PBS

Synonymes

242329D8, EC:1.1.1.205, IMP dehydrogenase 1, IMPD 1, IMPD1

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Galerie de données de validation

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  WB analysis using 84913-1-RR (same clone as 84913-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84913-1-RR (IMPDH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84913-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 84913-1-RR (same clone as 84913-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 84913-1-RR (IMPDH1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84913-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP01726-1

  The mean IMPDH1 concentration was determined to be 27.79 ng/mL in HeLa cell extract based on a 1.00 mg/mL extract load and 45.02 ng/mL in A431 cell extract based on a 1.30 mg/mL extract load.

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  Sandwich ELISA standard curve of MP01726-1

  Sandwich ELISA standard curve of MP01726-1, Human IMPDH1 Recombinant Matched Antibody Pair - PBS only. 84913-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag17473. 84913-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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  Affinity and Kinetic Characterization of 84913-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84913-1-RR against Human IMPDH1 were performed. The affinity constant is 4.41 nM.

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Informations sur le produit

84913-1-PBS cible IMPDH1 dans les applications de WB, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IMPDH1 Protéine recombinante Ag17473
• Nom complet: IMP (inosine monophosphate) dehydrogenase 1
• Masse moléculaire calculée: 563 aa, 60 kDa
• Poids moléculaire observé: 53-60 kDa
• Numéro d’acquisition GenBank: BC033622
• Symbole du gène: IMPDH1
• Identification du gène (NCBI): 3614
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IMPDH1(Inosine-5'-monophosphate dehydrogenase 1) is also named as IMPD1, IMP dehydrogenase 1 and belongs to the IMPDH/GMPR family. It catalyzes the rate limiting step of de novo guanine synthesis and an important target for the development of drugs with both chemotherapeutic and immunosuppressive activity. It may also have a role in the development of malignancy and the growth progression of some tumors. This protein has some isoforms produced by alternative splicing with the molecular mass of 53-65 kDa.

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