Anticorps Polyclonal de lapin anti-IMUP

• Phare
• Validé par KD/KO

IMUP Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 25561-1-AP

Synonymes

C19orf33, H2RSP, HAI 2 related small protein, IMUP, IMUP 1, IMUP 2

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Galerie de données de validation

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  WB analysis of A549 using 25561-1-AP

  WB result of IMUP antibody (25561-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IMUP transfected A549 cells.

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  WB analysis using 25561-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25561-1-AP (IMUP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 25561-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 25561-1-AP (C19orf33 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules EC109, cellules A549, cellules HeLa, cellules L02, cellules SGC-7901

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

25561-1-AP cible IMUP dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IMUP Protéine recombinante Ag22218
• Nom complet: chromosome 19 open reading frame 33
• Masse moléculaire calculée: 106 aa, 11 kDa
• Poids moléculaire observé: 20 kDa, 22 kDa
• Numéro d’acquisition GenBank: BC060319
• Symbole du gène: IMUP
• Identification du gène (NCBI): 64073
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Immortalization up-regulated protein (IMUP), also known as C19orf33 or H2RSP, was first identified in SV40-immortalized fibroblasts and includes two isoforms, IMUP-1 and IMUP-2 (PMID: 14981917; 35142956). It is found primarily in the nucleus (PMID: 11606055). IMUP is reportedly upregulated in many cancer cells and tissues and its overexpression is associated with tumorigenicity and cell-cycle acceleration (PMID: 16962562; 35142956; 22886722). This polyclonal antibody raised against 1-106 aa of human IMUP can recognize both IMUP-1 and IMUP-2. The apparent molecular determined by SDS-PAGE might be due to post-translational modifications or anomalous migration behavior (PMID: 11080599).

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