Anticorps Recombinant de lapin anti-IMUP

IMUP Recombinant Antibody for IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241220B4

N° de cat : 84148-6-PBS

Synonymes

Immortalization up-regulated protein, Hepatocyte growth factor activator inhibitor type 2-related small protein, HAI-2-related small protein, H2RSP, C19orf33

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Galerie de données de validation

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  IHC staining of human skin cancer using 84148-6-RR (same clone as 84148-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84148-6-RR (IMUP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84148-6-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 84148-6-RR (same clone as 84148-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84148-6-RR (IMUP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84148-6-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 84148-6-RR (same clone as 84148-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using C19orf33 antibody (84148-6-RR, Clone: 241220B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84148-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84148-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84148-6-RR against Human IMUP were performed. The affinity constant is 10.3 nM.

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Informations sur le produit

84148-6-PBS cible IMUP dans les applications de IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IMUP Protéine recombinante Ag22218
• Nom complet: chromosome 19 open reading frame 33
• Masse moléculaire calculée: 106 aa, 11 kDa
• Numéro d’acquisition GenBank: BC060319
• Symbole du gène: IMUP
• Identification du gène (NCBI): 64073
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Immortalization up-regulated protein (IMUP), also known as C19orf33 or H2RSP, was first identified in SV40-immortalized fibroblasts and includes two isoforms, IMUP-1 and IMUP-2 (PMID: 14981917; 35142956). It is found primarily in the nucleus (PMID: 11606055). IMUP is reportedly upregulated in many cancer cells and tissues and its overexpression is associated with tumorigenicity and cell-cycle acceleration (PMID: 16962562; 35142956; 22886722). The apparent molecular determined by SDS-PAGE might be due to post-translational modifications or anomalous migration behavior (PMID: 11080599).

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