Anticorps Recombinant de lapin anti-IMUP

IMUP Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241220A4

N° de cat : 84148-7-PBS

Synonymes

241220A4, C19orf33, H2RSP, HAI-2-related small protein, Hepatocyte growth factor activator inhibitor type 2-related small protein

---

Galerie de données de validation

• 

  WB analysis using 84148-7-RR (same clone as 84148-7-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84148-7-RR (IMUP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84148-7-PBS in a different storage buffer formulation.

• 

  FC experiment of A431 using 84148-7-RR (same clone as 84148-7-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug IMUP Recombinant antibody (84148-7-RR, Clone:241220A4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84148-7-PBS in a different storage buffer formulation.

• 

  FC experiment of PC-3 using 84148-7-RR (same clone as 84148-7-PBS)

  1x10^6 PC-3 cells were intracellularly stained with 0.25 ug IMUP Recombinant antibody (84148-7-RR, Clone:241220A4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84148-7-PBS in a different storage buffer formulation.

---

Informations sur le produit

84148-7-PBS cible IMUP dans les applications de WB, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IMUP Protéine recombinante Ag22218
• Nom complet: chromosome 19 open reading frame 33
• Masse moléculaire calculée: 106 aa, 11 kDa
• Poids moléculaire observé: 20-22 kDa
• Numéro d’acquisition GenBank: BC060319
• Symbole du gène: IMUP
• Identification du gène (NCBI): 64073
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Immortalization up-regulated protein (IMUP), also known as C19orf33 or H2RSP, was first identified in SV40-immortalized fibroblasts and includes two isoforms, IMUP-1 and IMUP-2 (PMID: 14981917; 35142956). It is found primarily in the nucleus (PMID: 11606055). IMUP is reportedly upregulated in many cancer cells and tissues and its overexpression is associated with tumorigenicity and cell-cycle acceleration (PMID: 16962562; 35142956; 22886722). The apparent molecular determined by SDS-PAGE might be due to post-translational modifications or anomalous migration behavior (PMID: 11080599).

{{ptg:RelatedPrimaryAntibodies}}