Anticorps Monoclonal anti-INA

INA Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3C12A8

N° de cat : 67120-1-PBS

Synonymes

Alpha internexin, 66 kDa neurofilament protein, NEF5, Alpha-Inx, Alpha-internexin

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Galerie de données de validation

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  WB analysis using 67120-1-Ig (same clone as 67120-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67120-1-Ig (INA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67120-1-PBS in a different storage buffer formulation.

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  WB analysis using 67120-1-Ig (same clone as 67120-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67120-1-Ig (INA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67120-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 67120-1-Ig (same clone as 67120-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67120-1-Ig (INA antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67120-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 67120-1-Ig (same clone as 67120-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67120-1-Ig (INA antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67120-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 67120-1-Ig (same clone as 67120-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using INA antibody (67120-1-Ig, Clone: 3C12A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67120-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67120-1-PBS cible INA dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: INA Protéine recombinante Ag13655
• Nom complet: internexin neuronal intermediate filament protein, alpha
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 60-66 kDa
• Numéro d’acquisition GenBank: BC006359
• Symbole du gène: INA
• Identification du gène (NCBI): 9118
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

INA, also named as Alpha internexin, is a 66 kDa intermediate filament protein which is involved in the morphogenesis of neurons. INA is mainly expressed in various kinds of central and peripheral neurons from early development and highly expressed in neurodegenerative and neuronal death cells. The MW of INA ranges from 60 kDa in human, pig and mouse to 66 kDa in rat.(PMID: 29940892, 2201753)

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