Anticorps Monoclonal anti-INF2

INF2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4A11A7

N° de cat : 66910-1-PBS

Synonymes

C14orf151, C14orf173, DKFZp762A0214, FLJ22056, HBEBP2 binding protein C, INF2, Inverted formin 2, pp9484

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Galerie de données de validation

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  WB analysis using 66910-1-Ig (same clone as 66910-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66910-1-Ig (INF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66910-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66910-1-Ig (same clone as 66910-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66910-1-Ig (INF2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66910-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66910-1-Ig (same clone as 66910-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66910-1-Ig (INF2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66910-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66910-1-Ig (same clone as 66910-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using INF2 antibody (66910-1-Ig, Clone: 4A11A7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66910-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66910-1-PBS cible INF2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: INF2 Protéine recombinante Ag17043
• Nom complet: inverted formin, FH2 and WH2 domain containing
• Masse moléculaire calculée: 1249 aa, 136 kDa
• Poids moléculaire observé: 150-170 kDa
• Numéro d’acquisition GenBank: BC008756
• Symbole du gène: INF2
• Identification du gène (NCBI): 64423
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

INF2 is a member of the formin family of actin-regulating proteins. INF2 is peripherally bound to the cytoplasmic face of the endoplasmic reticulum (ER). Mutations in the formin gene INF2 cause focal segmental glomerulosclerosis. The predicted MW of INF2 is around 136 kDa, while higher molecular mass from 140-200 kDa has been reported in literature (26383224,20493814,19366733).

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