Anticorps Polyclonal de lapin anti-ING5

• Phare
• Validé par KD/KO

ING5 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(28)

N° de cat : 10665-1-AP

Synonymes

Inhibitor of growth protein 5, p28ING5

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Galerie de données de validation

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  WB analysis of Jurkat using 10665-1-AP

  WB result of ING5 antibody (10665-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ING5 transfected Jurkat cells.

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  WB analysis of Jurkat using 10665-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10665-1-AP (ING5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10665-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10665-1-AP (ING5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10665-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10665-1-AP (ING5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 10665-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10665-1-AP (ING5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10665-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10665-1-AP (ING5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10665-1-AP

  IP result of anti-ING5 (IP:10665-1-AP, 4ug; Detection:10665-1-AP 1:700) with HEK-293 cells lysate 2560ug.

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  IHC staining of human colon cancer using 10665-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10665-1-AP (ING5 antibody) at dilution of 1:100 (under 25x lens).

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  IHC staining of human thyroid cancer using 10665-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10665-1-AP (ING5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 10665-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10665-1-AP (ING5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 10665-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 10665-1-AP (ING5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10665-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10665-1-AP (ING5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 10665-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10665-1-AP (ING5 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules PC-3, tissu rénal de souris
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu cutané de rat, tissu de cancer de la thyroïde humain, tissu de tumeur ovarienne humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 22 publications below
• IHC: See 10 publications below
• IF: See 5 publications below

Informations sur le produit

10665-1-AP cible ING5 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ING5 Protéine recombinante Ag1059
• Nom complet: inhibitor of growth family, member 5
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 28-32 kDa
• Numéro d’acquisition GenBank: BC005370
• Symbole du gène: ING5
• Identification du gène (NCBI): 84289
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ING5, also named as p28ING5, belongs to the ING family. It is a component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. ING5 is a component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Through chromatin acetylation it may regulate DNA replication and may function as a transcriptional coactivator. (PMID:12750254) Altered ING5 expression and its shift into the cytoplasm may have an impact on the malignant transformation of colorectal epithelial cells and should be considered as a biomarker for colorectal carcinogenesis. (PMID:21193223)

Publications

Species	Application	Title
rat	WB	Cell Res; LRP6 downregulation promotes cardiomyocyte proliferation and heart regeneration.; Authors - Yahan Wu ; KD Validated
mouse	WB	EMBO Rep; SCFJFK is functionally linked to obesity and metabolic syndrome.; Authors - Lin He
human	WB	Oncotarget; The miR-193a-3p-regulated ING5 gene activates the DNA damage response pathway and inhibits multi-chemoresistance in bladder cancer.; Authors - Yang Li
human	WB	Oncotarget; ING5 inhibits cancer aggressiveness via preventing EMT and is a potential prognostic biomarker for lung cancer.; Authors - Feng Zhang
human	IF	Oncotarget; ING5 suppresses proliferation, apoptosis, migration and invasion, and induces autophagy and differentiation of gastric cancer cells: a good marker for carcinogenesis and subsequent progression.; Authors - Wen-feng Gou ; KD Validated
human	WB,IHC	Oncotarget; Upregulated in Hepatitis B virus-associated hepatocellular carcinoma cells, miR-331-3p promotes proliferation of hepatocellular carcinoma cells by targeting ING5.; Authors - Yiyi Cao ; KD Validated

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