Anticorps Monoclonal anti-INO80

INO80 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F11C5

N° de cat : 68064-1-PBS

Synonymes

hINO80, EC:3.6.4.-, DNA helicase-related protein INO80, DNA helicase-related INO80 complex homolog 1, Chromatin-remodeling ATPase INO80

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Galerie de données de validation

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  WB analysis using 68064-1-Ig (same clone as 68064-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68064-1-Ig (INO80 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68064-1-PBS in a different storage buffer formulation.

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  WB analysis using 68064-1-Ig (same clone as 68064-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68064-1-Ig (INO80 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68064-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 68064-1-Ig (same clone as 68064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68064-1-Ig (INO80 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68064-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 68064-1-Ig (same clone as 68064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68064-1-Ig (INO80 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68064-1-PBS in a different storage buffer formulation.

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  FC experiment of PC-12 using 68064-1-Ig (same clone as 68064-1-PBS)

  1X10^6 PC-12 cells were intracellularly stained with 0.8 ug Anti-Human INO80 (68064-1-Ig, Clone:1F11C5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68064-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68064-1-PBS cible INO80 dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: INO80 Protéine recombinante Ag20265
• Nom complet: INO80 homolog (S. cerevisiae)
• Masse moléculaire calculée: 1556 aa, 177 kDa
• Poids moléculaire observé: 177 kDa
• Numéro d’acquisition GenBank: BC146785
• Symbole du gène: INO80
• Identification du gène (NCBI): 54617
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DNA helicase INO80, also namd as INO80 or hINO80, is a 1,556 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and one DBINO domain. INO80 co-localizes with PCMA at replication forks during S-phase in the nucleus and belongs to the SNF2 helicase family. INO80 is a component of the chromatin remolding INO80 complex and is widely expressed in many tissues, such as brain,liver and pancreas. INO80 is involved in transcription regulation.

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