Anticorps Polyclonal de lapin anti-INO80B

• Phare
• Validé par KD/KO

INO80B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29488-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 29488-1-AP (same clone as 29488-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 29488-1-AP (INO80B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29488-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 29488-1-AP (same clone as 29488-1-PBS)

  WB result of INO80B antibody (29488-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-INO80B transfected HeLa cells. This data was developed using the same antibody clone with 29488-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse ovary using 29488-1-AP (same clone as 29488-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 29488-1-AP (INO80B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29488-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse ovary using 29488-1-AP (same clone as 29488-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 29488-1-AP (INO80B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29488-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 29488-1-AP (same clone as 29488-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using INO80B antibody (29488-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 29488-1-PBS in a different storage buffer formulation.

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Informations sur le produit

29488-1-PBS cible INO80B dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: INO80B Protéine recombinante Ag30345
• Nom complet: INO80 complex subunit B
• Masse moléculaire calculée: 356 aa, 39 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC050666
• Symbole du gène: INO80B
• Identification du gène (NCBI): 83444
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

INO80B (INO80 complex subunit B), also known as ZNHIT4 (zinc finger HIT domain-containing protein 4), PAPA1 (PAP-1-associated protein 1), is a subunit of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. INO80B is localized to the nucleolus. The function of INO80B is to induce growth arrest by haulting the cell cycle at the G1 phase. INO80B expression is controlled at the transcriptional level and is highest at the G0 and G1 phases of the cell cycle. In vitro, INO80B binds PAP-1, a splicing factor, and may play a role in nucleolar complexes that regulate ribosome biogenesis and cell cycle events.

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