Anticorps Polyclonal de lapin anti-INPP5A

• Phare
• Validé par KD/KO

INPP5A Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 21723-1-AP

Synonymes

43 kDa inositol polyphosphate 5-phosphatase, 5PTASE, EC:3.1.3.56, Inositol polyphosphate-5-phosphatase A, Type I inositol 1,4,5-trisphosphate 5-phosphatase

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Galerie de données de validation

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  WB analysis of HeLa using 21723-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21723-1-AP (INPP5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 21723-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21723-1-AP (INPP5A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 21723-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21723-1-AP (INPP5A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 21723-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21723-1-AP (INPP5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 21723-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 21723-1-AP (INPP5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 21723-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21723-1-AP (INPP5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules COLO 320, cellules HeLa, cellules HepG2, tissu cérébral de rat, tissu cérébral humain

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

21723-1-AP cible INPP5A dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: INPP5A Protéine recombinante Ag16380
• Nom complet: inositol polyphosphate-5-phosphatase, 40kDa
• Masse moléculaire calculée: 412 aa, 48 kDa
• Poids moléculaire observé: 43-48 kDa
• Numéro d’acquisition GenBank: BC096280
• Symbole du gène: INPP5A
• Identification du gène (NCBI): 3632
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

INPP5A (Inositol Polyphosphate-5-Phosphatase A) is a membrane-bound type I inositol 1,4,5-trisphosphate 5-phosphatase that is involved in the mobilization of intracellular calcium ions and the regulation of cellular responses to stimuli. It terminates calcium signaling by hydrolyzing inositol 1,4,5-trisphosphate (InsP3) and inositol 1,3,4,5-tetrakisphosphate (InsP4). INPP5A plays important roles in various cellular processes, including cell proliferation, apoptosis, vesicular transport, and insulin signaling. Additionally, INPP5A is crucial for the survival of cerebellar Purkinje cells. In certain cancers, such as cutaneous squamous cell carcinoma (cSCC) and esophageal squamous cell carcinoma (ESCC), the expression levels of INPP5A are closely related to disease progression and prognosis.

Publications

Species	Application	Title
human	WB	Dev Cell; Functional genetic screen identifies ITPR3/calcium/RELB axis as a driver of colorectal cancer metastatic liver colonization.; Authors - Ryan H Moy ; KD Validated

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