Anticorps Monoclonal anti-INS

INS Monoclonal Antibody for IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4B6A7

N° de cat : 66198-1-PBS

Synonymes

insulin, 4B6A7

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Galerie de données de validation

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  IHC staining of human pancreas using 66198-1-Ig (same clone as 66198-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66198-1-Ig (Insulin Antibody) at dilution of 1:5000 (under 10x lens). This data was developed using the same antibody clone with 66198-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 66198-1-Ig (same clone as 66198-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66198-1-Ig (Insulin Antibody) at dilution of 1:5000 (under 40x lens). This data was developed using the same antibody clone with 66198-1-PBS in a different storage buffer formulation.

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  IHC staining of rat pancreas using 66198-1-Ig (same clone as 66198-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 66198-1-Ig (Insulin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66198-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat pancreas using 66198-1-Ig (same clone as 66198-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 66198-1-Ig (Insulin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66198-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 66198-1-Ig (same clone as 66198-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 66198-1-Ig (Insulin Antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 66198-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 66198-1-Ig (same clone as 66198-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 66198-1-Ig (Insulin Antibody) at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 66198-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human pancreas using 66198-1-Ig (same clone as 66198-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human pancreas tissue using 66198-1-Ig (Insulin antibody) at dilution of 1:100 and CoraLite594-Conjugated Goat Anti-Mouse IgG(H+L). The α cells of pancreas was labelled in green with 15954-1-AP (Glucagon antibody). This data was developed using the same antibody clone with 66198-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse pancreas using 66198-1-Ig (same clone as 66198-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using INS antibody (66198-1-Ig, Clone: 4B6A7 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L), Somatostatin antibody (24496-1-AP, green). This data was developed using the same antibody clone with 66198-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse pancreas using 66198-1-Ig (same clone as 66198-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using INS antibody (66198-1-Ig, Clone: 4B6A7 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66198-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse pancreas using 66198-1-Ig (same clone as 66198-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using INS antibody (66198-1-Ig, Clone: 4B6A7 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66198-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse pancreas using 66198-1-Ig (same clone as 66198-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using 66198-1-Ig (Insulin antibody), at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66198-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66198-1-PBS cible INS dans les applications de IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: INS Protéine recombinante Ag8630
• Nom complet: INS
• Masse moléculaire calculée: 110 aa, 12 kDa
• Numéro d’acquisition GenBank: BC005255
• Symbole du gène: INS
• Identification du gène (NCBI): 3630
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

INS is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. It participates in glucose utilization, protein synthesis and in the formation and storage of neutral lipids. INS is synthesized as a precursor molecule, pro-INS, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form INS, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Defects in INS results in type 1 diabetes mellitus. INS may also exist 36 kDa form corresponding to the hexameric INS form.

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