Anticorps Polyclonal de lapin anti-INSIG1

INSIG1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 55282-1-PBS

Synonymes

CL 6, INSIG 1, INSIG-1, insulin induced gene 1, Insulin induced gene 1 protein

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Galerie de données de validation

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  WB analysis using 55282-1-AP (same clone as 55282-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55282-1-AP (INSIG1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55282-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 55282-1-AP (same clone as 55282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55282-1-AP (INSIG1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55282-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 55282-1-AP (same clone as 55282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55282-1-AP (INSIG1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55282-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 55282-1-AP (same clone as 55282-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using INSIG1 antibody (55282-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 55282-1-PBS in a different storage buffer formulation.

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Informations sur le produit

55282-1-PBS cible INSIG1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: insulin induced gene 1
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: NM_005542
• Symbole du gène: INSIG1
• Identification du gène (NCBI): 3638
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Insulin-induced genes (INSIGs), which consist of two isoforms (INSIG1 and INSIG2), are representative endoplasmic reticulum (ER)-resident oxysterol-binding proteins (OSBPs). INSIG1 is part of negative regulatory feedback, acting as a brake on SREBP transcriptional function (PMID: 23919961). INSIG1 also has a secondary function mediating sterolaccelerated proteolytic degradation of HMG CoA reductase, thus impacting the mevalonate pathway. Suppression of INSIG1 function promotes hepatic lipid remodelling and restrains NASH (non-alcoholic steatohepatitis) progression(PMID: 33722690).

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