Anticorps Polyclonal de lapin anti-INSL3

INSL3 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 17997-1-AP

Synonymes

Insulin like 3, Insulin-like 3, Insulin-like 3 A chain, Insulin-like 3 B chain, Leydig insulin-like peptide

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Galerie de données de validation

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  WB analysis using 17997-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 17997-1-AP (INSL3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 17997-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17997-1-AP (INSL3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 17997-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17997-1-AP (INSL3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 17997-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17997-1-AP (INSL3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 17997-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17997-1-AP (INSL3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu testiculaire humain,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

17997-1-AP cible INSL3 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: INSL3 Protéine recombinante Ag12455
• Nom complet: insulin-like 3 (Leydig cell)
• Masse moléculaire calculée: 131 aa, 14 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC071706
• Symbole du gène: INSL3
• Identification du gène (NCBI): 3640
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Insulin-like 3 (INSL3), a Leydig cell-specific hormone, is essential for testis descent during foetal life and bone metabolism in adults. Insl3-deficient mice exhibit bilateral undescended testes located high in the abdominal cavity. INSL3 is presumed also to conform to a heterodimeric A-B structure of approximately 6 kDa once released from the producing cells. It is possible that both the mature form of INSL3 (6.6 kDa) and the pro-form(s) of INSL3 (12-14 kDa) are secreted from mammalian Leydig cells (PMID: 19329805).

Publications

Species	Application	Title
	WB	Exp Mol Pathol; Transcriptomic analysis of the HPT axis in a model of oligoasthenozoospermia induced by Adenine in rats; Authors - Nan Yang
rat	WB	Ecotoxicol Environ Saf; Identification of endocrine-disrupting chemicals targeting the genes and pathways of genital anomalies in males; Authors - Xiang Zhou

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