Anticorps Polyclonal de lapin anti-INSL3

INSL3 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 17997-1-PBS

Synonymes

Insulin like 3, Insulin-like 3, Insulin-like 3 A chain, Insulin-like 3 B chain, Leydig insulin-like peptide

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Galerie de données de validation

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  WB analysis using 17997-1-AP (same clone as 17997-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 17997-1-AP (INSL3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17997-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 17997-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17997-1-AP (INSL3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 17997-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17997-1-AP (INSL3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 17997-1-AP (same clone as 17997-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17997-1-AP (INSL3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17997-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 17997-1-AP (same clone as 17997-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17997-1-AP (INSL3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17997-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 17997-1-AP (same clone as 17997-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17997-1-AP (INSL3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17997-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 17997-1-AP (same clone as 17997-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17997-1-AP (INSL3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17997-1-PBS in a different storage buffer formulation.

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Informations sur le produit

17997-1-PBS cible INSL3 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: INSL3 Protéine recombinante Ag12455
• Nom complet: insulin-like 3 (Leydig cell)
• Masse moléculaire calculée: 131 aa, 14 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC071706
• Symbole du gène: INSL3
• Identification du gène (NCBI): 3640
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Insulin-like 3 (INSL3), a Leydig cell-specific hormone, is essential for testis descent during foetal life and bone metabolism in adults. Insl3-deficient mice exhibit bilateral undescended testes located high in the abdominal cavity. INSL3 is presumed also to conform to a heterodimeric A-B structure of approximately 6 kDa once released from the producing cells. It is possible that both the mature form of INSL3 (6.6 kDa) and the pro-form(s) of INSL3 (12-14 kDa) are secreted from mammalian Leydig cells (PMID: 19329805).

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