Anticorps Polyclonal de lapin anti-INSR

INSR Polyclonal Antibody for IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (5)

Applications

WB, IP, IHC, Dot blot, CoIP, ELISA

Conjugaison

Non conjugué

Publications

26

Avis (3)

N° de cat : 20433-1-AP

Synonymes

CD220, HHF5, INSR, IR

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Galerie de données de validation

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  WB analysis of HEK-293 using 20433-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20433-1-AP (INSR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 20433-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 20433-1-AP (INSR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 20433-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20433-1-AP (INSR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20433-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20433-1-AP (INSR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 20433-1-AP

  IP Result of anti-INSR (IP:20433-1-AP, 4ug; Detection:20433-1-AP 1:500) with HeLa cells lysate 1600ug.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HT-1080, cellules MCF-7
• Résultats positifs en IP: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 25 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

20433-1-AP cible INSR dans les applications de WB, IP, IHC, Dot blot, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, poisson-zèbre, porc, souris, fish
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: INSR Protéine recombinante Ag13922
• Nom complet: INSR
• Masse moléculaire calculée: 1382 aa, 156 kDa
• Poids moléculaire observé: 190 kDa, 135 kDa, 95 kDa
• Numéro d’acquisition GenBank: BC117172
• Symbole du gène: INSR
• Identification du gène (NCBI): 3643
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The biological effects of INS are mediated by a membrane spanning cell surface receptor that has been shown to consist of disulfide-linked 135-kDa and 95-kDa alpha- and beta-subunits that are cleaved products of a common precursor. Binding of INS to the receptor extracellular domain results in intracellular activation of a tyrosine-specific kinase activity and the generation of signals that determine the cellular response (PMID: 2369896). This antibody raised against 980-1382aa of human INS receptor can recognize full-length INS receptor precursor and INS receptor beta subunit.

Publications

Species	Application	Title
mouse	Dot blot	Environ Health Perspect; Effects of Chronic Secondhand Smoke (SHS) Exposure on Cognitive Performance and Metabolic Pathways in the Hippocampus of Wild-Type and Human Tau Mice.; Authors - Jacob Raber
rat	WB	Food Funct; l-Theanine regulates glucose, lipid, and protein metabolism via insulin and AMP-activated protein kinase signaling pathways; Authors - Ling Lin
human	WB	Br J Surg; MiR-128 regulation of glucose metabolism and cell proliferation in triple-negative breast cancer.; Authors - M Xiao
human	WB	FASEB J; Angptl7 promotes insulin resistance and type 2 diabetes mellitus by multiple mechanisms including SOCS3-mediated IRS1 degradation.; Authors - Tong Xu
mouse	WB,IHC	Sci Rep; NaoXinTong Capsules inhibit the development of diabetic nephropathy in db/db mice.; Authors - Shu Yang
mouse	WB	J Mol Med (Berl); Metabolomics reveals that chronic restraint stress alleviates carbon tetrachloride-induced hepatic fibrosis through the INSR/PI3K/AKT/AMPK pathway; Authors - Shanshan Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mi (Verified Customer) (05-29-2023)

It works well in human white adipocytes, we get strong signals

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: adipocytes

FH

Chiara (Verified Customer) (10-04-2021)

Works well but three bands

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: mesenchymal Stem cell

FH

Boyan (Verified Customer) (06-06-2021)

This antibody recognized a band at ~ 180 kd only, which is sensitive to PNGaseF, but not EndoH, treatment. The size is much bigger than an expected InsR band. Importantly, stripping the membrane and re-blot with another InsR antibody from a different brand could detect the protein at the expected MW. These results suggest this InsR antibody is detecting something other than InsR.

• Applications: Western Blot