Anticorps Recombinant de lapin anti-INSR/CD220

INSR/CD220 Recombinant Antibody for WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

241687H7

N° de cat : 84467-2-PBS

Synonymes

INSR, 241687H7, CD220, EC:2.7.10.1, HHF5

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Galerie de données de validation

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  WB analysis using 84467-2-RR (same clone as 84467-2-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 84467-2-RR (INSR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84467-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01349-2

  Sandwich ELISA standard curve of MP01349-2, Human INSR Recombinant Matched Antibody Pair - PBS only. 84467-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP00007. 84467-2-PBS was HRP conjugated as the detection antibody. Range: 1.56-100 ng/mL.

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  Sample test of MP01349-2

  The mean INSR concentration was determined to be 72.33 ng/mL in HEK-293 cell extract based on a 1.50 mg/mL extract load and 87.72 ng/mL in HT-1080 cell extract based on a 2.50 mg/mL extract load.

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  IHC staining of human breast cancer using 84467-2-RR (same clone as 84467-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 84467-2-RR (INSR/CD220 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84467-2-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 84467-2-RR (same clone as 84467-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using INSR/CD220 antibody (84467-2-RR, Clone: 241687H7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84467-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84467-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84467-2-RR against Human INSR were performed. The affinity constant is 0.189 nM.

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Informations sur le produit

84467-2-PBS cible INSR/CD220 dans les applications de WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: INSR
• Poids moléculaire observé: 95 kDa
• Numéro d’acquisition GenBank: BC117172
• Symbole du gène: INSR
• Identification du gène (NCBI): 3643
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The biological effects of INS are mediated by a membrane-spanning cell surface receptor that has been shown to consist of disulfide-linked alpha-subunits (135 kDa) and beta-subunits (95 kDa) that are cleaved products of a common precursor. The binding of INS to the receptor extracellular domain results in intracellular activation of a tyrosine-specific kinase activity and the generation of signals that determine the cellular response (PMID: 2369896).

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