Anticorps Recombinant de lapin anti-INSR/CD220

INSR/CD220 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

241687A10

N° de cat : 98227-1-RR

Synonymes

INSR, 241687A10, CD220, EC:2.7.10.1, HHF5

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Galerie de données de validation

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  FC experiment of human PBMCs using 98227-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human INSR/CD220 Rabbit Recombinant Antibody (98227-1-RR, Clone: 241687A10) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG. Cells were co-stained with CoraLite® Plus 647 Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  FC experiment of human PBMCs using 98227-1-RR

  1x10^6 FC receptor blocked human PBMCs were surface stained with 0.25 ug Anti-Human INSR/CD220 Rabbit Recombinant Antibody (98227-1-RR, Clone:241687A10) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98227-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98227-1-RR against Human INSR/CD220 were performed. The affinity constant is 52.4 pM.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98227-1-RR cible INSR/CD220 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: INSR
• Numéro d’acquisition GenBank: BC117172
• Symbole du gène: INSR
• Identification du gène (NCBI): 3643
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

The biological effects of INS are mediated by a membrane-spanning cell surface receptor that has been shown to consist of disulfide-linked alpha-subunits (135 kDa) and beta-subunits (95 kDa) that are cleaved products of a common precursor. The binding of INS to the receptor extracellular domain results in intracellular activation of a tyrosine-specific kinase activity and the generation of signals that determine the cellular response (PMID: 2369896).

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