Anticorps Recombinant de lapin anti-INTS5

INTS5 Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

230462B10

N° de cat : 83154-1-PBS

Synonymes

230462B10, Int5, INTS 5

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Galerie de données de validation

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  WB analysis using 83154-1-RR (same clone as 83154-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83154-1-RR (INTS5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83154-1-PBS in a different storage buffer formulation.

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  Sample test of MP00141-2

  The mean INTS5 concentration was determined to be 1.5 ng/mL in Hela cell extract based on a 1.1 mg/mL extract load.

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  IF Staining of U2OS using 83154-1-RR (same clone as 83154-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using INTS5 antibody (83154-1-RR, Clone: 230462B10 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83154-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83154-1-RR (same clone as 83154-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug INTS5 Recombinant antibody (83154-1-RR, Clone:230462B10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83154-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00141-2

  Cytometric bead array standard curve of MP00141-2, INTS5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83154-3-PBS. Detection antibody: 83154-1-PBS. Standard: Ag5129. Range: 0.625-20 ng/mL.

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  Affinity and Kinetic Characterization of 83154-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83154-1-RR against Human INTS5 were performed. The affinity constant is 0.968 nM.

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Informations sur le produit

83154-1-PBS cible INTS5 dans les applications de WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: INTS5 Protéine recombinante Ag5129
• Nom complet: integrator complex subunit 5
• Masse moléculaire calculée: 108 kDa
• Numéro d’acquisition GenBank: BC060841
• Symbole du gène: INTS5
• Identification du gène (NCBI): 80789
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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